Protein immunoassaying and purification

Details Protein immunoassaying and purification Protein immunoassaying and purification

1983-02-14

1986-05-20

Nucker, Christine M.

Chemistry: analytical and immunological testing

Involving an insoluble carrier for immobilizing immunochemicals

436531, 436532, 436512, 436547, 436513, 436818, 435810, 435 7, 422 61, 424 85, 530806, 530387, 530329, G01N 33543, C07K 517

Patent

active

045901686

ABSTRACT:
Protein immunoassay or purification using an antibody raised to an antigen comprising a polypeptide corresponding to a fragment (but not all) of the protein being assayed; the antibody is capable of binding separately both to the protein as a whole and to the polypeptide; that antibody is used to purify or (with a known concentration of the labeled polypeptide serving as a tracer) to assay the protein.

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patent: 4277437 (1981-07-01), Maggio
patent: 4465624 (1984-08-01), Chiba et al.
Merrifield, Biochem. (1964) 3 (No. 9): 1385-1390.
Lerner, Scientific American (1983) Feb.: 66-74.
Sherwood et al. (1979) Blood 54:885.
Miyake et al. (1977) J. Biological Chem. 252:5558.
Egrie, J., Hybridoma, vol. 2, No. 1, 1983, p. 136 "Monoclonal Antibodies to the N-Terminal Region of Hyman Erythropoietin."
Weis, T. L.; et al., Characterization of a Monoclonal Antibody to Erythropoietin, Proc. Natl. Acad. Sci. U.S.A., 79 (1982), pp. 5465-5469.

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