Protein immobilization on chelating resins

Details Protein immobilization on chelating resins Protein immobilization on chelating resins

1979-03-01

1981-01-20

Naff, David M.

Chemistry: molecular biology and microbiology

Carrier-bound or immobilized enzyme or microbial cell;...

Enzyme or microbial cell is immobilized on or in an organic...

260112R, 435 94, 435176, 435234, C12N 1108

Patent

active

042463505

ABSTRACT:
Proteins such as enzymes are immobilized on macroporous resins having recurring bis-picolylamine, imino diacetate or hydroxyethyl picolylamine chelating sites. Immobilization is carried out by contacting the resin with a multivalent metal ion selected from the transition metals or rare earth metals and adsorbing the protein to the resin. This immobilization technique permits strongly binding protein to a high surface area resin. The protein can be readily removed from the resin by simply flushing the resin with an appropriate solution.

REFERENCES:
patent: 3794563 (1974-02-01), Barker et al.
patent: 3912593 (1975-10-01), Barker et al.
patent: 3990943 (1976-11-01), Bouniot et al.
patent: 4025389 (1977-05-01), Poulson et al.
patent: 4098867 (1978-07-01), Grinstead et al.

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