Protein immobilization by adsorption of a hydrophobic amidine pr

Details Protein immobilization by adsorption of a hydrophobic amidine pr Protein immobilization by adsorption of a hydrophobic amidine pr

1986-09-05

1989-02-28

Naff, David M.

Chemistry: molecular biology and microbiology

Carrier-bound or immobilized enzyme or microbial cell;...

Enzyme or microbial cell is immobilized on or in an organic...

435177, 530815, C12N 1108, C12N 1102, C07K 1708

Patent

active

048085308

ABSTRACT:
Immobilization of biologically active protein is carried out by reacting the protein with an imidoester or imidothioester having a hydrophobic moiety to form a hydrophobic amidine derivative of the protein, and then adsorbing the protein derivative to a hydrophobic surface. The protein can be an enzyme such as trypsin and the hydrphobic surface can be a synthetic organic polymer.

REFERENCES:
patent: 3830699 (1974-09-01), Zaborsky
patent: 3969287 (1976-07-01), Jaworek et al.
patent: 4006059 (1977-02-01), Butler
patent: 4267273 (1981-05-01), Smith
Wu, et al., Biotech. & Bioeng, vol. XXIII, 1981, pp. 855-861.
Hunter, et al., J. Am. Chem. Soc. vol. 84, 1962, pp. 3491-3504.

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