Protein hydrolysates enriched in peptides having a carboxy...

Chemistry: molecular biology and microbiology – Enzyme – proenzyme; compositions thereof; process for... – Hydrolase

Reexamination Certificate

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Details

C435S320100, C435S254110, C426S063000, C536S023200, C536S023740

Reexamination Certificate

active

10450185

ABSTRACT:
The present invention provides An isolated polypeptide which has proline specific endoprotease activity, selected from the group consisting of:(a) a polypeptide which has an amino acid sequence which has at least 40% amino acid sequence identity with amino acids 1 to 526 of SEQ ID NO:2 or a fragment thereof;(b) a polypeptide which is encoded by a polynucleotide which hybridizes under low stringency conditions with (i) the nucleic acid sequence of SEQ ID NO:1 or a fragment thereof which is at least 80% or 90% identical over 60, preferably over 100 nucleotides, more preferably at least 90% identical over 200 nucleotides, or (ii) a nucleic acid sequence complementary to the nucleic acid sequence of SEQ ID NO:1.

REFERENCES:
patent: 0223 560 (1987-05-01), None
patent: 321 603 (1989-06-01), None
patent: 325 986 (1989-08-01), None
patent: 0 522 428 (1993-01-01), None
patent: 5015314 (1993-01-01), None
patent: WO 96/13174 (1996-05-01), None
Database accession No. BE759716, XP002203451.
Habibi-Najafi and Lee, Journal of Dairy Science (1994) 77(2):385-392.

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