Protein having pesticidal activity, DNA encoding the...

Chemistry: natural resins or derivatives; peptides or proteins; – Proteins – i.e. – more than 100 amino acid residues

Reexamination Certificate

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C530S300000, C435S007100, C435S069100, C424S093200, C424S093200, C514S012200, C514S002600

Reexamination Certificate

active

06962977

ABSTRACT:
The invention provides a noxious organism-controlling agent effective to control pests that have acquired a resistance to conventional Bt agents, and which has activity onColeopterapests. Also, the invention provides a microbe,Bacillus thuringiensis serovar galleriaeSDS502 strain, that produces a toxic protein. This toxic protein can serve as the active ingredient in a noxious organism-controlling composition. The invention provides a protein having pesticidal activity that is produced by theBacillus thuringiensis serovar galleriaeSDS502 strain, and a protein having an addition, deletion or substitution of a plurality of amino acids and having similar pesticidal activity. The invention also provides a DNA encoding protein having pesticidal activity, a microbe transformed with the DNA, and a plant transformed with the DNA and its seed.

REFERENCES:
patent: 5554534 (1996-09-01), Michaels et al.
patent: 498537 (1992-08-01), None
patent: 06-065292 (1994-03-01), None
patent: 07-000179 (1995-01-01), None
patent: 08-228783 (1996-09-01), None
patent: WO 92/19106 (1992-11-01), None
patent: WO 93/04587 (1993-03-01), None
Wells, Biochemistry, vol. 29, pp. 8509-8517, 1990.
International Search Report.
Ryoichi Sato, et al., “Cloning, heterologous expression, and localization of a novel crystal protein gene fromBacillus thurigiensusserovar japonensis strain buibui toxic to scaarabaeid insects”, Current Microbiology (1994), vol. 28, No. 1, pp. 15-19.
Patent Abstracts of Japan, JP-06-065292, dated Mar. 8, 1994.
Patent Abstracts of Japan, JP-07-000179, dated Jan. 6, 1995.
Patent Abstracts of Japan, JP08-228783, dated Sep. 10, 1996.

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