Protein having nitrile hydratase activity obtained from rhizobiu

Details Protein having nitrile hydratase activity obtained from rhizobiu Protein having nitrile hydratase activity obtained from rhizobiu

1993-04-27

1994-05-24

Wax, Robert A.

Chemistry: molecular biology and microbiology

Enzyme , proenzyme; compositions thereof; process for...

Lyase

435 691, 435129, 435 712, 4352523, 43525233, 4351723, 4353201, 935 14, 935 29, 935 56, 935 72, 935 73, 536 232, C12N 988, C12N 1560, C12N 1570

Patent

active

053148193

ABSTRACT:
A novel water-soluble enzyme protein being composed of three heterogeneous subunits and having nitrile hydratase activity was separated and purified from the bacterium of genus Rhizobium. The nucleotide sequence of the gene encoding the protein was determined; using the transformant transformed with the expression vector having the nucleotide sequence, the protein was expressed; by reacting the transformant with a nitrile in a culture broth, the corresponding amide was produced.
Via the use of a culture broth containing the transformant described above or the protein itself, a nitrile can be efficiently converted into the corresponding amide.

REFERENCES:
patent: 5130235 (1992-07-01), Beppu et al.
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J. Bacteriol., 172, 6764-6773 (1990).
J. Bacteriol., 173, 2465-2472 (1991).
J. Bacteriol., 173, 6694-6704 (1991).
Biochimica et Biophysica Acta, 1088, 225-233 (1991).
Biochimica et Biophysica Acta, 1129, 23-33 (1991).
Eur. J. Biochem., 196, 581-589 (1991).
J. Gen. Microbiol., 138, 1003-1010 (1992).

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