Chemistry: molecular biology and microbiology – Enzyme – proenzyme; compositions thereof; process for... – Oxidoreductase
Patent
1997-04-07
1998-04-28
Hendricks, Keith D.
Chemistry: molecular biology and microbiology
Enzyme , proenzyme; compositions thereof; process for...
Oxidoreductase
435189, C12N 904
Patent
active
057443424
ABSTRACT:
A novel protein having a heat-resistant malate dehydrogenase activity, a DNA fragment having a gene encoding said protein, a recombinant vector having said DNA fragment, a transformant transformed with said vector, a method for producing the protein having heat-resistant malate dehydrogenase activity by the use of said transformant, a reagent for GOT determination, comprising the above-mentioned novel protein having a heat-resistant malate dehydrogenase activity and a method for determining GOT activity, which comprises the use of said reagent. According to the present invention, a protein having a heat-resistant malate dehydrogenase activity and having higher purity and superior heat stability can be obtained. In addition, a reagent for GOT determination which is superior in long-term storage can be prepared by the use of the protein having a heat-resistant malate dehydrogenase activity.
REFERENCES:
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Iijima et al., "Physicochemical and Catalytic Properties of Thermostable Malate Dehydrogenase From an Extreme Thermophile Thermus Flavus AT-62", Biochimica et Biophysica Acta, 613, 1-9 (1980).
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Wilks et al. "A Specific, Highly Active Malate Dehydrogenase by Redesign of a Lactate Dehydrogenase Framework", Science, 242, 1541-1544 (1988).
Wright et al. "Simple Efficient Methods for the Isolation of Malate Dehydrogenase from Thermophilic and Mesophilic Bacteria", Biochem. J., 177, 441-448 (1979).
You et al., "Purification and Properties of Malate Dehydrogenase from Pseudomonas testosteroni", Journal of Bacteriology, 123, No. 2, 704-716 (1975).
Emi Shigenori
Kawamura Yoshihisa
Sogabe Atsushi
Takeshima Seiji
Teshima Shin'ichi
Hendricks Keith D.
Toyo Boseki Kabushiki Kaisha
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