Protein having heat-resistant malate dehydrogenase activity

Details Protein having heat-resistant malate dehydrogenase activity Protein having heat-resistant malate dehydrogenase activity

1994-07-01

1997-11-11

Hendricks, Keith D.

Chemistry: molecular biology and microbiology

Micro-organism, per se ; compositions thereof; proces of...

Bacteria or actinomycetales; media therefor

4353201, 43525231, 43525233, 435190, 536 232, C12N 1553, C12N 904

Patent

active

056862946

ABSTRACT:
A novel protein having a heat-resistant malate dehydrogenase activity, a DNA fragment having a gene encoding said protein, a recombinant vector having said DNA fragment, a transformant transformed with said vector, a method for producing the protein having heat-resistant malate dehydrogenase activity by the use of said transformant, a reagent for GOT determination, comprising the above-mentioned novel protein having a heat-resistant malate dehydrogenase activity and a method for determining GOT activity, which comprises the use of said reagent. According to the present invention, a protein having a heat-resistant malate dehydrogenase activity and having higher purity and superior heat stability can be obtained. In addition, a reagent for GOT determination which is superior in long-term storage can be prepared by the use of the protein having a heat-resistant malate dehydrogenase activity.

REFERENCES:
Birkraft et al., "The Presence of a Histidine-Aspartic Acid pair in the Active Site of 2-Hydroxyacid Dehydrogenases", J. Biol. Chem., 258: 472 (1983).
Sundaram et al., "Malate Dehydrogenase from Thermophilic and Mesophilic Bacteria Molecular Size, Subunit Structure, Amino Acid Composition, Immunochemical Homology, and Catalytic Activity", Biochemistry, 19, 2017-2022 (1980).
Murphey et al., "Purification and Propertieis of Bacillus Subtilis, Bacillus Stearothermophilus, and Escherichia Coli Malate Dehydrogenases", The Journal of Biological Chemistry, 242, No. 7, 1548-1559 (1967).
Rolstad et al., "Malate Dehydrogenase from the Thermophilic Green Bacterium Chloroflexus aurantiacus: Purification, Molecular Weight, Amino Acid Composition, and Partial Amino Acid Sequence", Journal of Bacteriology, 170 No. 7, 2947-2953 (1988).
Ohshima et al., "Purification and Characterization of Malate Dehydrogenase from the Phototrophic Bacterium, Rhodopseudomonas Capsulata", Biochimica et Biophysica Acta, 869, 171-177 (1986).
You et al., "Purification and Properties of Malate Dehydrogenase from Pseudomonas Testosterni", Journal of Bacteriology, 123, No. 2, 704-716 (1975).
Iijima et al., "Physiochemical and Catalytic Properties of Thermostable Malate Dehydrogenase From an Extreme Thermophile Thermus Flavus at 62", Biochemica et Biophysica Acta, 613, 1-9 (1980).
Lepock et al. Biochimica et Biophysica Acta, 1990, vol. 1055, pp. 19-26.
Wright et al. Biochem J., 177:441-448, 1979.
Wilks et al., Science, vol. 242, pp. 1541-1544, 16 Dec. 1988.
Sambrook et al. Molecular Sloning: A Laboratory Manual, 1989, Cold Spring Harbor Press, pp. 11-3-11.19.

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