Protein glycosylation modification in methylotrophic yeast

Details Protein glycosylation modification in methylotrophic yeast Protein glycosylation modification in methylotrophic yeast

2007-08-07

2007-08-07

Slobodyansky, Elizabeth (Department: 1652)

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving nucleic acid

C435S193000, C435S203000, C435S254110, C435S254230, C435S320100, C536S023200

Reexamination Certificate

active

10185475

ABSTRACT:
The present invention provides genetically engineered strains of methylotrophic yeast includingPichiaand especiallyPichia pastoriscapable of producing proteins with reduced or modified glycosylation. Methods of producing glycoproteins with reduced and/or modified glycosylation using such genetically engineered strains ofPichiaare also provided. Vectors, which comprise coding sequences for α-1,2-mannosidase I, glucosidase II, GlcNAc-tranferase I and mannosidase II or comprising OCH1 disrupting sequence, for transforming methylotrophic yeasts are contemplated by the present invention. Kit for providing the comtemplated vectors are also included in this invention.

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