Protein-free nutrient medium

Details Protein-free nutrient medium Protein-free nutrient medium

1977-08-01

1979-01-09

Tanenholtz, Alvin

Textiles: fiber preparation

195102, 2603474, 195 96, C12K 110

Patent

active

041337174

ABSTRACT:
A process for the preparation of a special protein-free nutrient medium having unexpected efficiency in the propagation of Leptospira. The process comprises strategically admixing a modified or detoxified polysorbate fatty acid to a preformed mixture containing vitamin B.sub.12, vitamin B.sub.1, soluble manganese, magnesium and ammonium salts, and a buffer, in a water diluent adjusted to pH 6.5 to 8.5. The polysorbate fatty acid is modified or detoxified by admixture with activated charcoal followed by settling, decantation, centrifugation and filtration to remove all charcoal particles.

REFERENCES:
patent: 3816261 (1974-06-01), Torney

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