Protein-free defined media for the growth of normal human...

Details Protein-free defined media for the growth of normal human... Protein-free defined media for the growth of normal human...

2006-05-02

2006-05-02

Lankford, Jr., Leon B. (Department: 1651)

Chemistry: molecular biology and microbiology

Animal cell, per se ; composition thereof; process of...

Culture medium, per se

C435S405000, C435S383000, C435S384000, C435S325000

Reexamination Certificate

active

07037721

ABSTRACT:
Improvements are made to a novel media that replace the requirement for all protein growth factors by the addition to the medium of physiological concentrations of retinyl acetate. The media are serum-free, companion cell or feeder layer-free and organotypic, matrix free solutions for the cultivation of clonally competent basal keratinocytes. The media and methods are useful in the production of epidermal epithelial tissue that is suitable for skin grafting.

REFERENCES:
patent: 5753506 (1998-05-01), Johe
patent: 6030789 (2000-02-01), Ward et al.

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