Protein-free culture medium which promotes hybridoma growth

Details Protein-free culture medium which promotes hybridoma growth Protein-free culture medium which promotes hybridoma growth

1989-08-15

1991-09-03

Stone, Jacqueline

Chemistry: molecular biology and microbiology

Spore forming or isolating process

43524027, 4352403, C12N 502, C12N 512

Patent

active

050454684

ABSTRACT:
A chemically-defined, stable, protein-free medium has been devised which is capable of supporting the growth of lymphoid cells, particularly hybridomas, even in anchorage-independent cultures. Doubling rates are close to those obtained with serum-supplemented media. The medium preferably comprises a nonproteinaceous organo-iron compound, especially a nitroprusside salt, an iron chelator such as EDTA, and a selenium compound, such as selenium dioxide or sodium selenite.

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