Protein-free culture medium

Details Protein-free culture medium Protein-free culture medium

1983-10-07

1988-08-30

Wiseman, Thomas G.

Chemistry: molecular biology and microbiology

Micro-organism, tissue cell culture or enzyme using process...

Enzymatic production of a protein or polypeptide

43524025, 43524027, 4352403, 43524031, 435948, C12P 2100, C12N 500, C12N 502, C12R 191

Patent

active

047677040

ABSTRACT:
A trace element mixture suitable for use in a protein-free tissue culture medium, which comprises water-soluble compounds selected from acids, bases, and salts containing copper, iron, zinc, manganese, silicon, molybdenum, vanadium, nickel, tin, aluminum, silver, barium, bromine, cadmium, cobalt, chromium, fluorine, germanium, iodine, rubidium, zirconium, or selenium, the compounds being devoid of any additional metals other than those present as positive ions selected from groups IA and IIA of the periodic table of elements, wherein the compounds produce a solution containing specified minimum concentrations of the listed elements when dissolved in an amount of water sufficient to produce a concentration for one of the elements equal to the corresponding minimum concentration of the one element while maintaining each remaining element at a concentration equal to or greater than the minimum concentration for the remaining element is disclosed along with cell culture media containing these trace elements and methods of culturing cells using these media.

REFERENCES:
Reinert, J. and M. M. Yeoman, Plant Cell and Tissue Culture, NY, Springer-Verlag, 1982, pp. 77-79.
McKeehan, W. L., K. A. McKeehan, S. L. Hammond and R. G. Ham, In Vitro, 13(7), 399-416, 1977.
Chang et al., "Production of Monoclonal Antibodies in Serum Free Medium", J. of Immunological Methods, vol. 39, pp. 369-375, 1980.
Hamilton, Clonal Growth of Chinese Hamster Cell Lines", 1977, In Vitro, vol. 13, No. 7, pp. 537-547.
Cleveland et al., J. of Immunological Methods, vol. 56, pp. 221-234, 1983.
Mosier, "Primary In Vitro Antibody Responses", 1981, J. of Immunology, vol. 127, No. 4, pp. 1490-1493.
Schroeder et al., Arch. Environ. Health; vol. 23, Aug. 1971, pp. 102-106.
Titanium, World Health Organization, Geneva, 1982, pp. 12-49.
James N. Ihle et al., Journal of Immunology, 126, Jun. 1981, pp. 2184-2189.
Kearney et al., Journal of Immunology, 123, Oct. 1979, pp. 1548-1550.
Yelton et al., Hybridoma, 1, 1981, pp. 5-11.
Laskov and Scharff, J. Exp. Med., 131, 1970, pp. 515-541.
Benjamin et al., Proc. Natl. Acad. Sci. USA, 79, 1982, pp. 5379-5383.
Kincade et al., J. of Immunological Methods, 42, 1981, pp. 17-24.
Marshak-Rothstein et al., J. of Immunology, 122, 1979, pp. 2491-2497.

Affiliated with

Also associated with

Rating

Protein-free culture medium does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Protein-free culture medium, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Protein-free culture medium will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFUS-PAI-O-2088028