Protein fragment complementation assays in whole animals...

Details Protein fragment complementation assays in whole animals... Protein fragment complementation assays in whole animals...

2007-12-11

2007-12-11

Brusca, John S (Department: 1631)

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving nucleic acid

C536S023400

Reexamination Certificate

active

10882292

ABSTRACT:
The instant invention describes a method for detecting protein-protein interactions in living organisms and/or cells, said method comprising: (a) synthesizing probe protein fragments from a protein which enables fluorescent or luminescent detection by dissecting the gene coding for the fluorescent or luminescent protein into a least two fragments; (2) constructing fusion proteins consisting of the probe protein fragments linked to protein domains that are to be tested for interactions; (3) coexpressing the fusion proteins; and (d) detecting reconstitution of the fluorescence or luminescence signal.

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Fields et al. The two-hybrid system: an assay for protein-protein interactions. Trends in Genetics vol. 10, pp. 286-292 (1994).
Johnson et al. Split ubiquitin as a sensor of protein interactions in vivo. Proc. Natl. Acad. Sci. USA vol. 91, pp. 10340-10344 (1994).

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