Protein fragment complementation assays for the detection of...

Details Protein fragment complementation assays for the detection of... Protein fragment complementation assays for the detection of...

2007-01-09

2007-01-09

Brusca, John S. (Department: 1631)

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving luciferase

C435S069100

Reexamination Certificate

active

10353090

ABSTRACT:
The present invention describes a method for detecting biomolecular interactions said method comprising: (a) selecting an appropriate reporter molecule selected from the group consisting of a protein, a fluorescent protein, a luminescent protein and a phosphorescent protein; (b) effecting fragmentation of said reporter molecule such that said fragmentation results in reversible loss of reporter function; (c) fusing or attaching fragments of said reporter molecule separately to other molecules; followed by (d) reassociation of said reporter fragments through interactions of the molecules that are fused to said fragments; and (e) detecting said biomolecular interactions by reconstitution of activity of the reporter molecule.

REFERENCES:
patent: 6270964 (2001-08-01), Michnick et al.
patent: 6294330 (2001-09-01), Michnick et al.
patent: 6428951 (2002-08-01), Michnick et al.
patent: 6897017 (2005-05-01), Michnick et al.
patent: 6929916 (2005-08-01), Michnick et al.

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