Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving nucleic acid
Reexamination Certificate
2005-08-16
2005-08-16
Brusca, John S. (Department: 1631)
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving nucleic acid
C435S320100, C530S350000, C536S023400
Reexamination Certificate
active
06929916
ABSTRACT:
The present invention describes a method for detecting biomolecular interactions said method comprising: (a) selecting an appropriate reporter molecule selected from the group consisting of a protein, a fluorescent protein, a luminescent protein and a phosphorescent protein; (b) effecting fragmentation of said reporter molecule such that said fragmentation results in reversible loss of reporter function; (c) fusing or attaching fragments of said reporter molecule separately to other molecules; followed by (d) reassociation of said reporter fragments through interactions of the molecules that are fused to said fragments; and (e) detecting said biomolecular interactions by reconstitution of activity of the reporter molecule with the proviso that said protein is not ubiquitin.
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Fields et al. The two-hybrid system: an assay for protein-protein interactions. Trends in Genetics vol. 10 pp. 286-292 (1994).
Johnsson et al. Split ubiquitin as a sensor of protein interactions in vivo. Proc. Natl. Acad. Sci. vol. 91 pp. 10340-10344.
Michnick Stephen William Watson
Pelletier Joelle Nina
Remy Ingrid
Angres Isaac A.
Brusca John S.
Odyssey Thera Inc.
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