Protein fragment complementation assays for the detection of...

Details Protein fragment complementation assays for the detection of... Protein fragment complementation assays for the detection of...

2011-08-02

2011-08-02

Cheu, Jacob (Department: 1641)

Chemistry: analytical and immunological testing

Involving an insoluble carrier for immobilizing immunochemicals

C435S007100

Reexamination Certificate

active

07989218

ABSTRACT:
The present invention describes a method for detecting biomolecular interactions said method comprising: (a) selecting an appropriate reporter molecule selected from the group consisting of a protein, a fluorescent protein, a luminescent protein and a phosphorescent protein; (b) effecting fragmentation of said reporter molecule such that said fragmentation results in reversible loss of reporter function; (c) fusing or attaching fragments of said reporter molecule separately to other molecules; followed by (d) reassociation of said reporter fragments through interactions of the molecules that are fused to said fragments; and (e) detecting said biomolecular interactions by reconstitution of activity of the reporter molecule.

REFERENCES:
Evangelista et al. (Trends in Cell Biology 1996 vol. 6, p. 196-199).

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