Education and demonstration – Detection or range determination of distant object by... – Radar
Reexamination Certificate
2006-06-13
2006-06-13
Brusca, John S. (Department: 1631)
Education and demonstration
Detection or range determination of distant object by...
Radar
C530S350000, C536S023400
Reexamination Certificate
active
07062219
ABSTRACT:
The present invention provides protein single-color and multi-color protein fragment complementation assays for drug discovery, in particular to identify compounds that activate or inhibit cellular pathways. Based on the selection of an interacting protein pair combined with an appropriate PCA reporter such as monomeric enzymes and fluorescent proteins, the assays may be run in high-throughput or high-content mode and may be used in automated screening of libraries of compounds. Methods are described for constructing such assays for one or more steps in a biochemical pathway; testing the effects of compounds from combinatorial, natural product, peptide, antibody, nucleic acid or other diverse libraries on the protein or pathway(s) of interest; and using the results of the screening to identify specific compounds that activate or inhibit the protein or pathway(s) of interest. The development of such assays provides for a broad, flexible and biologically relevant platform for drug discovery.
REFERENCES:
patent: 6270964 (2001-08-01), Michnick et al.
patent: 6294330 (2001-09-01), Michnick et al.
patent: 6428951 (2002-08-01), Michnick et al.
Panayotou et al. The assembly of signaling complexes by receptor tyrosine kinases. BioEssays, vol. 15, No. 3 pp. 171-177 (1993).
Ko et al. p53: puzzle and paradigm Genes and Development, vol. 10, pp. 1054-1072 (1996).
Johnsson et al. Split ubiquitin as a sensor of protein interactions in vivo. Proc. Natl. Acad. Sci. USA vol. 91, pp. 10340-10344 (1994).
Lamerdin Jane
MacDonald Marnie
Michnick Stephen William Watson
Remy Ingrid
Westwick John K.
Angres Issac A.
Brusca John S.
Odyssey Thera Inc.
LandOfFree
Protein fragment complementation assays for high-throughput... does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Protein fragment complementation assays for high-throughput..., we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Protein fragment complementation assays for high-throughput... will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-3706269