Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Recombinant dna technique included in method of making a...
Reexamination Certificate
2004-06-25
2011-11-29
Desai, Anand (Department: 1656)
Chemistry: molecular biology and microbiology
Micro-organism, tissue cell culture or enzyme using process...
Recombinant dna technique included in method of making a...
C530S300000
Reexamination Certificate
active
08067198
ABSTRACT:
The present invention relates to a method of producing a target protein, which method comprises expressing said protein in a host cell which contains a nucleic acid molecule which encodes a chimeric protein, said chimeric protein comprising a signal peptide from a non-mammalian bulk-secreted protein and said target protein; nucleic acids, vectors, host cells and kits for carrying out the method are also described.
REFERENCES:
patent: 5876995 (1999-03-01), Bryan
patent: 6066781 (2000-05-01), Sutliff et al.
patent: 6113886 (2000-09-01), Bryan
patent: 6152358 (2000-11-01), Bryan
patent: 6232107 (2001-05-01), Bryan et al.
patent: 6247995 (2001-06-01), Bryan
patent: 6416960 (2002-07-01), Bryan
patent: 6436682 (2002-08-01), Bryan et al.
patent: 2002/0004942 (2002-01-01), Bryan
patent: 2003/0066096 (2003-04-01), Bryan
patent: 0 266 057 (1988-05-01), None
patent: 0 279 582 (1988-08-01), None
patent: 2001-258565 (2001-09-01), None
patent: WO 87/03300 (1987-06-01), None
patent: WO 91/13151 (1991-09-01), None
patent: WO 93/04165 (1993-03-01), None
patent: WO 97/29319 (1997-08-01), None
patent: WO 98/36081 (1998-08-01), None
patent: WO 98/36085 (1998-08-01), None
patent: WO 99/13090 (1999-03-01), None
patent: WO 99/49010 (1999-09-01), None
patent: WO 99/49019 (1999-09-01), None
patent: WO 00/26366 (2000-05-01), None
patent: WO 00/50616 (2000-08-01), None
patent: WO 02/46430 (2002-06-01), None
Allet et al. (1997) Protein Expression and Purification, vol. 9, pp. 61-68.
Brumell et al.,“SopD2 is a novel type III secreted effector of Salmonella typhimurium that targets late endocytic compartments upon delivery into host cells”, Traffic 4: 36-48 (Jan. 2003).
EBI Accession No. EM-PRO: U89490 (Sequence Listing).
EBI Accession No. UNIPROT: Q9BLZ2 (Sequence Listing).
Hesketh et al., “Intracellular Sorting of Macromolecules,” Biochemical Society Transactions, vol. 24 No. 2 pp. 521-527, (1996).
Huang and Yen, “Hepatitis B Virus RNA Element That Facilitates Accumulation of Surface Gene Transcripts in the Cytoplasm,” Journal of Virology, vol. 68, No. 5, pp. 3193-3199, (May 1994).
Inouye S. et al., “Imaging of luciferase secretion from transformed Chinese hamster ovary cells,” Proc. Natl. Acad. Sci., vol. 89, pp. 9584-9587, (Oct. 1992).
Instruction Manual Entitled “pSecTag2/Hygro A, B, and C,” Catalog No. V910-20.
Kim et al., “Molecular Cloning and Expression of Human Galβ1,3GaINAc α2,3-Sialytransferase (hST3Gal II),” Biochemical and Biophysical Research Communication, vol. 228, pp. 324-327, (1996).
Maeda et al., “Efficient Production of Active TNF-α by Albumin Signal Peptide,” Biochemistry and Molecular Biology International, vol. 42, No. 4, pp. 825-832, (Jul. 1997).
Miesenböck and Rothman, “Patterns of synaptic activity in neural networks recorded by light emission from synaptolucins,” Proc. Natl. Acad. Sci., vol. 94, pp. 3402-3407, (Apr. 1997).
Partridge et al., “Competition between the signal sequence and a 3'UTR localization signal during redirection of beta-globin mRNA to the endoplasmic reticulum: implications for biotechnology,” Cytotechnology, vol. 30, pp. 37-47, (1999).
Thompson et al., “Vargula hilgendorfiiluciferase: a secreted reporter enzyme for monitoring gene expression in mammalian cells,” Gene, vol. 96:2, pp. 257-262, (1990).
Thompson et al., “Cloning and expression of cDNA for the luciferase from the marine ostracodVargula hilgendorfii,” Proc. Natl. Acad. Sci., vol. 86, pp. 6567-6571; (Sep. 1989).
Veyrune et al., “A localization signal in the 3' untranslated region of c-mycmRNA targets c-mycmRNA and β-globin reporter sequences to the perinuclear cytoplasm and cytoskeletal-bound polysomes,” Journal of Cell Science, vol. 109, pp. 1185-1194, (1996).
Zambetti et al., “Targeting of a chimeric human histone fusion mRNA to membrane-bound polysomes in HeLa cells,” Proc. Natl. Acad. Sci., vol. 84, pp. 2683-2687, (May 1987).
Ahn, J.O., et al Over Expression of Thermoalkalophilic Lipase fromBacillus stearothermophilusL1 inSaccharomyces cerevisiae, J. Microbiol. Biotechnol. (2003) 13(3), 451-456.
International Search Report issued May 9, 2005 for PCT/GB2004/002779 filed on Jun. 25, 2004, which published on Jan. 6, 2005 as WO 2005/001099 (Hesketh et al. listed as inventors and Unitargeting Research AS listed as Applicant).
International Preliminary Report on Patentability and Written Opinion issued Jan. 3, 2006 for PCT/GB2004/002779 filed on Jun. 25, 2004, which published on Jan. 6, 2005 as WO 2005/001099 (Hesketh et al. listed as inventors and Unitargeting Research AS listed as Applicant).
Amatruda JF, Gattermeir DJ, Karpova TS, Cooper JA. (1992) Effects of null mutations and overexpression of capping protein on morphogenesis, actin distribution and polarized secn in yeast. J Cell Biol. 119(5): 1151-1162.
Erratt JA, Douglas PE, Moranelli F, Seligy VL. (1984) The induction of alpha-amylase by starch inAspergillus oryzae: evidence for controlled mRNA expression. Can J Biochem Cell Biol. 62(8): 678-690.
Jennes W, Vereecken C, Fransen K, de Roo A, Kestens L. (2004) Disturbed secretory capacity for macrophage inflammatory protein (MIP)-1 alpha and MIP-1 beta in progressive HIV infection. AIDS Res Hum Retroviruses. 20(10): 1087-1091.
Kaneko M, Itoh H, Ueguchi-Tanaka M, Ashikari M, Matsuoka M. (2002) The alpha-amylase induction in endosperm during rice seed germination is caused by gibberellin synthesized in epithelium. Plant Physiol. 128(4): 1264-1270.
Kolarow R, Brigadski T, Lessmann V. (2007) Postsynaptic secretion of BDNF and NT-3 from hippocampal neurons depends on calcium calmodulin kinase II signaling and proceeds via delayed fusion pore opening. J Neurosci. 27(39): 10350-10364.
Lessmann V, Gottmann K, Malcangio M. (2003) Neurotrophin secretion: current facts and future prospects. Prog Neurobiol. 69(5): 341-374.
Liu G, Greenshields DL, Sammynaiken R, Hirji RN, Selvaraj G, Wei Y. (2007) Targeted alterations in iron homeostasis underlie plant defense responses. J Cell Sci. 120(Pt 4): 596-605.
Perrin D, Möller K, Hanke K, Söling HD. (1992) cAMP and Ca(2+)-mediated secretion in parotid acinar cells is associated with reversible changes in the organization of the cytoskeleton. J Cell Biol. 116(1): 127-134.
Kamiya T, Sugio S, Yamanouchi K, Kagitani Y. (1996) Secretion of active Fc fragments of immunoglobulin E directed by the yeast invertase signal sequence in mammalian cells. Tohoku J Exp Med. 180(4): 297-308.
Communication pursuant to Article 94(3) EPC issued Jun. 8, 2010 for EP Application No. 04 743 127.5-2405, which EP application claims priority to PCT/GB2004/002779 filed on Jun. 25, 2004 (Hesketh et al. listed as inventors and Prolume, Ltd. as Applicant).
Response to Communication filed Feb. 4, 2009 for EP Application No. 04 743 127.5-2405, which EP application claims priority to PCT/GB2004/002779 filed on Jun. 25, 2004 (Hesketh et al. listed as inventors and Prolume, Ltd. as Applicant).
Communication pursuant to Article 94(3) EPC issued Jul. 25, 2008 for EP Application No. 04 743 127.5-2405, which EP application claims priority to PCT/GB2004/002779 filed on Jun. 25, 2004 (Hesketh et al. listed as inventors and Prolume, Ltd. as Applicant).
Response to Communication filed Jan. 14, 2008 for EP Application No. 04 743 127.5-2405, which EP application claims priority to PCT/GB2004/002779 filed on Jun. 25, 2004 (Hesketh et al. listed as inventors and UniTargeting Research AS as Applicant).
Loss of Rights pursuant to R. 69(1) EPC issued on Nov. 2, 2007 for EP Application No. 04 743 127.5-2405, which EP application claims priority to PCT/GB2004/002779 filed on Jun. 25, 2004 (Hesketh et al. listed as inventors and UniTargeting Research AS as Applicant).
Communication pursuant to Article 96(2) EPC issued Mar. 15, 2007 for EP Application No. 04 743 127.5-2405, which EP application claims priority to PCT/GB2004/002779 filed on Jun. 25, 2004 (Hesketh et al. listed as inventors and UniTarget
Gjerdrum Christine
Hesketh John
Pryme Ian
Ravneberg Hanne
Stern Beate
Ballard Spahr LLP
Desai Anand
Prolume Ltd.
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