Chemistry: natural resins or derivatives; peptides or proteins; – Carrier - bound or immobilized peptides or proteins and the... – Peptides or proteins is immobilized on – or in – an organic...
Patent
1996-01-16
1998-09-15
Railey, II, Johnny F.
Chemistry: natural resins or derivatives; peptides or proteins;
Carrier - bound or immobilized peptides or proteins and the...
Peptides or proteins is immobilized on, or in, an organic...
525 541, C07K 1704, C07K 1708
Patent
active
058080120
ABSTRACT:
Thermoplastics interdispersed with a variety of functional thermostable proteins and methods for their production are provided. To prepare the subject thermoplastics, a plastic material is contacted with a thermostable polypeptide and then subjected to the heating and molding/extrusion/casting process. The resultant thermoplastics comprise the thermostable polypeptide on the formed plastic surface and at a depth below the plastic surface. The thermostable polypeptides contained in the disclosed compositions retain functional properties or binding specificities through the heating and molding/extrusion/casting processes. Preferred thermostable polypeptides used in the disclosed compositions include silk-like protein polymers, particularly ProNectin.RTM.F. The disclosed methods and compositions find use in many applications where plastics containing finctional thermostable proteins are desired, in particular, cell cultureware.
REFERENCES:
patent: 4617271 (1986-10-01), Nambu
Dale and McBennett, "Stability of High-Temperature Enzymes," ACS Symp. Ser. (1992), 498:136-152.
Finkelstein & Reva, "A Search for the Most Stable Folds of Protein Chains", Nature (1991), 351:497-499.
Goodenough and Jenkins, "Protein Engineering to Change Thermal Stability for Food Enzymes," Biochem. Soc. Trans. (1991), 19:655-662.
Lee and Lee, "Thermal Stability of Proteins in the Presence of Poly(ethylene glycols)," Biochemistry (1987), 26:7813-7819.
MacLeod et al., "Stabilization of Proteins to Heat," Research Disclosure (1984), 244:380.
Matthews et al., "Enhanced Protein Thermostability from Site-directed Mutations that Decrease the Entropy of Unfolding," PNAS USA (1987), 84:6664-6667.
Ponnuswamy et al., "Amino Acid Composition and Thermal Stability of Proteins", Int. J. Biol. Macromol (1982), 4:186-190.
Santoro et al., "Increased Thermal Stability of Proteins in the Presence of Naturally Occurring Osmolytes," Biochemistry (1992), 31:5278-5283.
Wampler et al., "Computational Approaches to Modeling and Analyzing Thermostablity in Proteins," ACS Symp. Ser. (1992), 498:153-173.
Wood and Gadow, "Immobilization of Antibodies and Antigens on macro Solid Phases--A Comparison Between Adsorptive and Covalent Binding", J. Clin.. Chem. Biochem. (1983), 21:789-797.
Wozniak et al., "Crystallographic and Genetic Approaches Toward the Design of Proteins of Enhanced Thermostability," Crystallogr. Model Methods Mol. Des. (1990), Ed. Bugg and Ealick, NY, NY, 80-94.
Donofrio David M.
Stedronsky Erwin R.
Kresnak Mark T.
Protein Polymer Technologies, Inc.
Railey II Johnny F.
Trecartin Richard F.
LandOfFree
Protein-enriched thermoplastics does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Protein-enriched thermoplastics, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Protein-enriched thermoplastics will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-88883