Chemistry: analytical and immunological testing – Peptide – protein or amino acid
Patent
1987-01-22
1988-11-01
Richman, Barry S.
Chemistry: analytical and immunological testing
Peptide, protein or amino acid
436169, 436174, 436905, 530417, 530344, 530395, G01N 3300
Patent
active
047820276
ABSTRACT:
A polypeptide is located in a gel matrix by treating the matrix with a negative staining agent (i.e. an agent that stains the gel more intensely in regions devoid of polypeptides, so that gel areas exhibiting reduced staining intensity are indicative of the presence of polypeptide). Specifically, the staining agent comprises a copper or cobalt salt, and a detergent. The term polypeptide is used to include polypeptides of all sizes, including proteins and glycoproteins. The polypeptide can be purified by applying it to a gel matrix, causing it to run into the matrix, applying the above-described staining agent and then eluting the polypeptides from the matrix.
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Lee Christopher
Levin Andrew
Alexander Lyle A.
President and Fellows of Harvard College
Richman Barry S.
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