Protein-deamidating enzyme, microorganism producing the...

Chemistry: molecular biology and microbiology – Enzyme – proenzyme; compositions thereof; process for... – Hydrolase

Reexamination Certificate

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C435S004000, C435S006120, C435S069100, C435S071100, C435S018000, C435S041000, C435S252300, C435S320100, C536S023200

Reexamination Certificate

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07569378

ABSTRACT:
A method for the production of an enzyme, which comprises culturing in a medium a strain that belongs to a bacterium classified into Cytophagales or Actinomycetes, or a new bacteriumChryseobacteriumsp. No. 9670 belonging to the genusChryseobacterium, and has the ability to produce an enzyme having a property to deamidate amido groups in protein, thereby effecting production of the enzyme, and subsequently collecting the enzyme from the culture mixture and a method for the modification of protein making use of a novel enzyme which directly acts upon amido groups in protein, as well as a gene which encodes the enzyme, a recombinant vector which contains the gene, a transformant transformed with the vector and a method in which the transformant is cultured in a medium to effect production of the protein-deamidating enzyme and then the protein-deamidating enzyme is collected from the culture mixture.

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patent: 0976829 (2000-02-01), None
patent: WO-97/43910 (1997-11-01), None
Vaintraub et al., Protein Deamidase from Germinating Wheat Grains, FEBS Letts vol. 302, No. 2 (May 1992), pp. 169-171.
Yamaguchi et al., A Novel Protein-Deamidating Enzyme from Chryseobacterium Proteolyticum SP. Nov., a Newly Isolated Bacterium from Soil, Appl. Environ. Microbiol. vol. 66, No. 8, (Aug. 2000), pp. 3337-3343.
Hamada et al., Preparation and Functional Properties of Enzymatically Deamidated Soy Proteins, J. Food. Sci. vol. 54, No. 3, (May 1989), pp. 598-601, 635.
Database WPI, Section Ch, Week 199922, Derwent Publications Ltd., London, GB; AN 1999-257687, XP002162641 & JP 11 075835 (Amano Pham KK), Mar. 23, 1999 abstract.
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