Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving transferase
Reexamination Certificate
2007-10-09
2007-10-09
Rao, Manjunath (Department: 1652)
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving transferase
C435S004000, C435S006120, C435S069100, C435S183000, C435S200000, C435S211000, C536S023200
Reexamination Certificate
active
10815751
ABSTRACT:
A novel enzyme which has an activity to release side chain carboxyl groups and ammonia from a protein by acting upon side chain amido groups in the protein. This invention relates to a method for the production of an enzyme, which comprises culturing in a medium a strain that belongs to a bacterium classified intoCytophagalesorActinomycetesand has the ability to produce an enzyme having a property to deamidate amido groups in protein, thereby effecting production of said enzyme, and subsequently collecting said enzyme from the culture mixture. It also relates to a method for the modification of protein making use of a novel enzyme which directly acts upon amido groups in protein as well as to an enzyme which has a property to deamidate amido groups in protein and a gene which encodes said enzyme.
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I.A. Vaintraub, et al., “Protein Deamidase from Germinating Wheat Grains”,FEBS Letters,vol. 302, No. 2, May 11, 1992, pp. 169-171.
Yamaguchi, et al., “A Novel Protein-Deamidating Enzyme fromChryseobacterium proteolyticumSP SP. Nov. , a Newly Isolated Bacterium from Soil”,Appl. Environ, Microbiol.,vol. 66, No. 8, Aug. 2000, pp. 3337-3343. (XP-002162600).
DATABASE WPI, Section Ch, Week 199922, Derwent Publications Ltd., London, GB; Class B04, AN 1999-257687, XP002162641 & JP 11 075835 (Amano Pharm KK), Mar. 23, 1999.
Matsuura Akira
Yamaguchi Shotaro
Amano Pharmaceutical Co. Ltd.
Edwards Angell Palmer & & Dodge LLP
Rao Manjunath
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