Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving nucleic acid
Patent
1997-11-05
1999-01-12
Housel, James C.
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving nucleic acid
536 237, 536 243, 536 2432, 536 2433, 4242561, 435 732, C12Q 168
Patent
active
058586776
ABSTRACT:
A novel surface exposed protein of H. influenzae or related Haemophilus species is described. The protein named protein D is an Ig receptor for human IgD and has an apparent molecular weight of 42,000. Protein D can be detected in all of 116 encapsulated and non-encapsulated isolates of H. influenzae studied. The protein from all strains shows in addition to the same apparent molecular weight immunogenic similarities since protein D from all strains interacts with three different mouse monoclonal antibodies and monoclonal human IgD. A method for purification of protein D is described. Cloning of the protein D gene from H. influenzae in E. coli is described as well as the nucleotide sequence and the deduced amino acid sequence corresponding to a molecular weight of 41,821 daltons including a putative signal sequence of 18 amino acids containing a consensus sequence (SEQ ID NO: 2), Leu-Ala-Gly-Lys for bacterial lipoproteins.
REFERENCES:
patent: 3800798 (1974-04-01), Winkler
patent: 3995018 (1976-11-01), Sjoquist
"Molecular Analysis of DNA and Construction of Genomic Libraries of Mycobacterium leprae", Josephine E. Clark-Curtiss et al, Journal of Bacteriology, vol. 161, No. 3, pp. 1093-1102, 1985.
"The 3'-Terminal Sequence of Escherichia coli 16S Ribosomal RNA: Complementarity to Nonsense Triplets and Ribosome Binding Sites", J. Shine et al, Proc. Nat. Acad. Sci, USA, vol. 71, No. 4, pp. 1342-1346, 1974.
"Effects of the Complete Removal of Basic Amino Acid Residues from the Signal Peptide on Secretion of Lipoprotein in Escherichia coli*", The Journal of Biological Chemistry, George P. Vlasuk et al, vol. 258, No. 11, pp. 7141-7148, 1983.
"Many Bacterial Species Bind Human IgD.sup.1 ", Arne Forsgren et al., The Journal of Immunology, vol. 122, No. 4, pp. 1468-1472, 1979.
"Protein D, the Immunoglobulin D-Binding Protein of Haemophilus influenzae, Is a Lipoprotein", H.ang.kan Janson et al., Infection and Immunity, vol. 60, No. 4, Apr. 1992, pp. 1336-1342.
"Nontypable Haemophilus influenzae: A Review of Clinical Aspects, Surface Antigens, and the Human Immune Response to Infection", Timothy E. Murphy et al., Review of Infectious Diseases, vol. 9, No. 1, Jan.-Feb. 1987 pp. 1-15.
Infection and Immunity, vol. 59, No. 1, pp. 119 to 125, H.ang.ken Janson et al. (Jan. 1991), "Protein D, an Immunoglobulin D-Binding Protein of Haemophilus influenzae: Cloning, Nucleotide Sequence, and Expression in Escherichia coli".
The Journal of Immunology, vol. 145, No. 10, pp. 3379 to 3384, Maorong Ruan et al. (Nov. 15, 1990), "Protein D of Haemophilus influenzae A Novel Bacterial Surface Protein with Affinity for Human IgD.sup.1 ".
The Journal of Immunology, vol. 122, No. 4, pp. 1468 to 1472, Arne Forsgren et al., "Many Bacterial Species Bind Human IgD.sup.1 ".
Safety, Tolerability, and Immunogenicity of Concurrent Administration of Haemophilus influenzae Type b Conjugate Vaccine (Meningococcal Protein Conjugate) with Either Measles-Mumps-Rubella Vaccine or Diphtheria-Tetanus-Pertussis and Oral Poliovirus Vaccines in 14-to 23-Month Old Infants, Barry Dashefsky et al., Supplement to Pediatrics, American Academy of Pediatrics, Elk Grove Village, Illinois 60009-0927, Apr. 1990, vol. 85, No. 4, Part 2, pp. 682-689.
"Protein D, a Putative Immunoglobulin D-Binding Protein Produced by Haemophilus influenzae, Is Glycerophosphodiester Phosphodiesterase", Robert S. Munson, Jr. et al., Journal of Bacteriology, Jul. 1993, vol. 175, No. 14, pp. 4569-4571.
"Protein of Mice Against the Lethal Effect of an Intraperitoneal Infection with Haemophilus (Actinobacillus) pleuropneumoniae after Vaccination with Capsular Proteins", D.K. Lenser et al., Veterinary Medicine, 18 (1988) pp. 335-348.
"Purification and Characterization of glpQ-Encoded Glycerophosphodiester Phosphodiesterase from Escherichia coli K-12", Timothy J. Larson et al., Archives of Biochemistry and Biophysics, vol. 260, No. 2, Feb. 1, 1988, pp. 577-584.
"Protein D of Haemophilus influenzae Is Not a Universal Immunoglobulin D-Binding Protein", Ken Sasaki et al., Infection and Immunity, Jul. 1993, vol. 61, No. 7, pp. 3026-3031.
"Efficacy of Cell Extract from Actinobacillus Haemophilus pleuropneumoniae Serotype 1 Against Disease in Swine", P.I. Fedorka et al., Infection and Immunity, Feb. 1990, vol. 58, No. 2, pp. 358-365.
"Pneumococcal Conjugate Vaccines", The Pediatric Infectious Disease Journal, Workshop on Vaccines for Otitis Media, MR Loeb et al., vol. 8, No. 1 Supplement, Jan. 1989, S48-S50.
"High Degrees of Conservation of Protein D Genes from Nontypable and Type b Strains of Haemophilus influenzae", H. Janson et al., Abstract of the General Meeting--1992, American Society Microbiology, May 26-30, 1992, 92:136.
"Protection of Infant Rats from Haemophilus influenzae Type b Infection by Antiserum to Purified Outer Membrane Protein a", Marilyn R. Loeb, Infection and Immunity, vol. 55, No. 11, pp. 2612-2618, 1987.
"Purification and Comparison of Outer Membrane Protein P2 from Haemophilus influenzae Type b Isolates", Robert S. Munson, Jr. et al., J. Chem. Invest., vol. 72, pp. 677-684, 1983.
"Purification and Partial Characterization of Outer Membrane Proteins P5 and P6 from Haemophilus influenzae Type b", Robert S. Munson, Jr. et al., Infection and Immunity, vol. 49, pp. 544-549, 1985.
Alan Kimura et al, "A Minor High-Molecular-Weight Outer Membrane Protein of Haemophilus influenzae Type b is a Protective Antigen", Infection and Immunity, vol. 47, No. 1, pp. 253-259, 1985.
Timothy F. Murphy et al, "A Subtyping System for Nontypable Haemophilus influenzae Based on Outer-Membrane Proteins", The Journal of Infectious Diseases, vol. 147, No. 5, pp. 838-846, 1983.
Klaus Weber et al, "The Reliability of Molecular Weight Determinations by Dodecyl Sulfate-Polyacrylamide Gel Electrophoresis", The Journal of Biological Chemistry, vol. 244, No. 16, pp. 4406-4412, 1969.
Housel James C.
Shaver Jennifer
LandOfFree
Protein D--an IgD-binding protein of haemophilus influenzae does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Protein D--an IgD-binding protein of haemophilus influenzae, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Protein D--an IgD-binding protein of haemophilus influenzae will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-1514118