Protein crystal growth apparatus for microgravitiy

Chemical apparatus and process disinfecting – deodorizing – preser – Physical type apparatus – Crystallizer

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117200, 117202, 422100, B01D 900

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056435401

ABSTRACT:
Apparatus for growing protein crystals under microgravity environment includes a plurality of protein growth assemblies stacked one above the other within a canister. Each of the protein growth assemblies includes a tray having a number of spaced apart growth chambers recessed below an upper surface, the growth chambers each having an upstanding pedestal and an annular reservoir about the pedestal for receiving a wick and precipitating agents. A well is recessed below the top of each pedestal to define a protein crystal growth receptacle. A flexible membrane is positioned on the upper surface of each tray and a sealing plate is positioned above each membrane, each sealing plate having a number of bumpers corresponding in number and alignment to the pedestals for forcing the membrane selectively against the upper end of the respective pedestal to seal the reservoir and the receptacle when the sealing plate is forced down.

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McPherson, "Preparation and Analysis of Protein Crystals," 1982, Chapter 4, pp. 82-127.
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Miller, et al., "A comparison between protein crystals grown with vapor diffusion methods in microgravity and protein crystals using a gel liquid-liquid diffusion ground based method," Journal of Crystal Growth 122 (1992), pp. 306-309, North-Holland, Amsterdam.
Gernert, et al., "A Simple Apparatus for Controlling Nucleation and Size in Protein Crystal Growth," Analytical Biochemistry 168, pp. 141-147 (1988), U.S.A.

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