Protein crystal comprising the processivity clamp factor of...

Chemistry: molecular biology and microbiology – Enzyme – proenzyme; compositions thereof; process for...

Reexamination Certificate

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C530S350000, C702S019000

Reexamination Certificate

active

07635583

ABSTRACT:
A protein crystal having the processivity clamp factor of DNA polymerase that is the β subunit of DNA polymerase III ofEscherichia coliand a peptide of about 3 to about 30 amino acids, in particular of about 16 amino acids. The peptide includes all or part of the processivity clamp factor binding sequence of a processivity clamp factor interacting protein, such as prokaryotic Pol I, Pol II, Pol III, Pol IV, Pol V, MutS, ligase I, α subunit of DNA polymerase, UmuD or UmuD′, or eukaryotic pol ε, pol δ, pol η, pol ι, pol κ.

REFERENCES:
patent: WO0238596 (2002-05-01), None
patent: WO 03/025004 (2003-03-01), None
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Giege et al., Crystallogenesis of Biological Macromolecule: Facts and Perspectives, (1994) Acta Cryst., vol. D50, pp. 339-350.
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Drenth, Principles of Protein X-ray Crystallography (1995) Springer, New York, p. 1.
Kierzek et al., Models of protein crystal growth, (2001) Biophys Chem, 91:1-20.
Wiencek, New Strategies for Protein Crystal Growth, (1999) Ann Rev Biomed Eng., 1:505-534.
Kong X-P et al: “The-dimensional structure of the beta subunit ofE. coliDNA polymerase III holenzyme: a sliding DNA clamp”, Cell, Cell Press, Cambridge, NA, US, vol. 69, May 1, 1992, pp. 425-437, XP002968224, ISSN: 0092-8674, the whole document.
Yang Wei:, “Damage repair DNA polymerases Y.”, Current Opinion in Structural Biology, vol. 13, No. 1, Feb. 2003, pp. 23-30, XP002262979, ISSN: 0959-440X (ISSN pring) the whole document.
Podobnik Marjetka et al:, “Nucleotide -induced conformational changes in an isolatedEscherichia coliDNA polymerase III clamp loader subunit.”, Structure (Cambridge), vol. 11, No. 3, Mar. 2003, pp. 253-263, XP002262980, ISSN: 0969-2126 (ISSN pring) the whole document.
Jeruzalmi David et al:, “Crystal structure of the processivity clamp loader gamma (gamma) complex ofE. coliDNA polymerase III”, Cell vol. 106, No. 4, Aug. 24, 2001, pp. 429-441, XP002262981, ISSN: 0092-8674, the whole document.

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