Drug – bio-affecting and body treating compositions – Designated organic active ingredient containing – Peptide containing doai
Patent
1995-05-16
2000-05-09
Patterson, Jr., Charles L.
Drug, bio-affecting and body treating compositions
Designated organic active ingredient containing
Peptide containing doai
530383, G07K 14255, A61K 3837
Patent
active
060604476
ABSTRACT:
Recombinant protein complexes having human Factor VIII:C activity are expressed in a eukaryotic host cell by transforming the host cell with first and second expression cassettes encoding a first polypeptide substantially homologous to human Factor VIII:C A domain and a second polypeptide substantially homologous to human Factor VIII:C C domain, respectively. In the present invention, the first polypeptide may be extended having at its C-terminal a human Factor VIII:C B domain N-terminal peptide, a polypeptide spacer of 3-40 amino acids, and a human Factor VIII:C B domain C-terminal peptide. Expression of the second polypeptide is improved by employing an .alpha..sub.1 -antitrypsin signal sequence.
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Burke Rae Lyn
Chapman Barbara
Mikkelson Jan Moller
Rasmussen Mirella Ezban
Blackburn Robert P.
Chiron Corporation
Guth Joseph H.
Novo Nordisk A S
Patterson Jr. Charles L.
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