Protein cleavage method and use thereof

Details Protein cleavage method and use thereof Protein cleavage method and use thereof

2006-05-02

2010-10-26

Weber, Jon P (Department: 1657)

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving hydrolase

Reexamination Certificate

active

07820404

ABSTRACT:
The present invention provides a method for cleaving a glycated protein to obtain an amino acid or a peptide efficiently with a protease. By treating the glycated protein with the protease in the presence of a compound represented by R—X, the amino acid or the peptide is obtained by the cleavage. The R represents an alkyl compound with a carbon number of 9 or more, and preferably is straight-chain alkyl or straight-chain acyl with a carbon number of 9 to 16, branched-chain alkyl or branched-chain acyl with a carbon number of 10 to 40 and a main-chain carbon number of 9 to 16, or straight-chain alkyl that is substituted by cycloalkyl (a carbon number of the cycloalkyl ranges from 3 to 8, and a carbon number of the straight chain ranges from 4 to 13), where X is a sugar residue. Moreover, the glycated protein is, for example, glycated hemoglobin, and preferably β-chain N-terminal amino acid or a β-chain N-terminal peptide is cleaved by the protease treatment.

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