Protein C production in non-human transgenic mammals

Details Protein C production in non-human transgenic mammals Protein C production in non-human transgenic mammals

1996-11-26

1999-05-18

Crouch, Deborah

Multicellular living organisms and unmodified parts thereof and

Nonhuman animal

Transgenic nonhuman animal

800 4, 800 21, 435 691, 935 60, A01K67/027

Patent

active

059051850

ABSTRACT:
Methods for producing protein C in transgenic non-human mammals are disclosed. The protein C is modified at the two-chain cleavage site between the light and heavy chains of protein C from Lys-Arg to R.sub.1 -R.sub.2 -R.sub.3 -R.sub.4 where R.sub.1 through R.sub.4 are individually Arg or Lys. DNA segments encoding modified protein C are introduced into the germ line of a non-human mammals, and the mammal or its female progeny produces milk containing protein C expressed from the introduced DNA segments. The protein C expressed from the introduced DNA segments has anticoagulant activity when activated. Non-human mammalian embryos and transgenic non-human mammals carrying DNA segments encoding heterologous protein C are also disclosed.

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