Protein-assay reagent and method

Details Protein-assay reagent and method Protein-assay reagent and method

1976-06-10

1977-05-17

Reese, Robert M.

Chemistry: physical processes

Physical processes

Crystallization

252408, G01N 3316

Patent

active

040239330

ABSTRACT:
A dye binding reagent for protein assay comprises Coomassie Brilliant Blue G-250 and an acid having a pKa of from 1 to 2. Upon addition of the reagent to a protein-containing solution, the attendant color change can be spectrophotometrically measured to quantitate even micrograms of protein. Furthermore, the reagent is applicable to a wide range of proteins and requires only about 2 minutes for measurement. Addition of an alcohol such as ethanol to the reagent further improves sensitivity.

REFERENCES:
patent: 2897058 (1959-07-01), Galat
patent: 3873272 (1975-03-01), Wakefield et al.
patent: 3884637 (1975-05-01), Gindler
patent: 3936271 (1976-02-01), Statter

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