Protein assay by silver binding

Details Protein assay by silver binding Protein assay by silver binding

1984-12-17

1985-11-19

Turk, Arnold

Chemistry: analytical and immunological testing

Peptide, protein or amino acid

436164, 436175, G01N 2178, G01N 3368

Patent

active

045542546

ABSTRACT:
A novel, inexpensive, highly sensitive and quantitative assay for measuring protein in solution based on the capacity of protein to bind silver is described. In this procedure, protein samples are first treated with glutaraldehyde and then exposed to ammoniacal silver. After a specified time, the reaction is terminated by the addition of sodium thiosulfate and the optical density measured at 420 nm. The useful range of the assay for the majority of standard proteins tested lies between 15 and 2000 ng. This represents a 100-fold increase in sensitivity over the Coomassie Brilliant Blue dye binding procedure. There is little or no interference from carbohydrates, non-ionic detergents or ethanol. Pretreatment of protein samples with Bio-Gel P-2 to remove salts, thiol agents, EDTA and SDS, makes this procedure compatible with most commonly used buffers.

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