Protein A-silica immunoadsorbent and process for its production

Details Protein A-silica immunoadsorbent and process for its production Protein A-silica immunoadsorbent and process for its production

1985-01-11

1987-07-21

Garvin, Patrick P.

Catalyst, solid sorbent, or support therefor: product or process

Solid sorbent

Organic

128DIG3, 210263, 210691, B01J 2022

Patent

active

046818707

ABSTRACT:
An immunoadsorbent material for removing IgG and IgG-complexes from biological fluids is prepared by covalently binding protein A to a solid-phase silica matrix. It has been found that particularly stable, high-capacity immunoadsorbents are obtained by derivatizing the silica with amino and/or carboxyl groups, and reacting the protein A with a carbodiimide at a pH in the range from 3.5 to 4.5. Binding through free hydroxyl groups may be achieved with cyanogen halides at a pH in the range from 11.0 to 11.5. After acid washing (pH 2.0-2.5) to remove non-covalently bound protein A, the immunoadsorbent may be employed in a column for therapeutic treatment of various cancers and autoimmune disorders where IgG-complexes are implicated as suppressing factors in inhibiting a normal immune response.

REFERENCES:
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"Affinity Chromatography--Principles and Methods" published by Pharmacia Fine Chemicals, Uppsala, Sweden.
Howard H. Weetall (1976) Meth. Enzymol. 44:134-148.

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