Chemistry: molecular biology and microbiology – Vector – per se
Reexamination Certificate
2005-02-01
2005-02-01
Graser, Jennifer E. (Department: 1645)
Chemistry: molecular biology and microbiology
Vector, per se
C536S023700, C536S024100, C536S024200
Reexamination Certificate
active
06849447
ABSTRACT:
Protective high molecular weight (HMW) proteins are produced recombinantly by expression fromE. coliby using a promoter effective inE. coliand a nucleic acid molecule which contains a modified operon of a non-typeable strain of Haemophilus. The modified operon contains the portion only of the A region which encodes the mature HMW protein and the complete B and C regions of the operon. Enhanced levels of expression of the HMW proteins can be achieved by including theE. colicer gene, a further copy of the portion of the A region of the operon encoding the mature protein or both in the expression vector. Nucleotide and deduced amino acid sequences of the hmw1 and hmw2 genes and HMW1 and HMW2 proteins, respectively of several non-typeableHaemophilus influenzaestrain have been identified.
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Klein Michel H.
Loosmore Sheena M.
Yang Yan-Ping
Aventis Pasteur Limited
Graser Jennifer E.
Sim & McBurney
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