Protection from viral infection via colonization of mucosal memb

Drug – bio-affecting and body treating compositions – Whole live micro-organism – cell – or virus containing

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424 932, 424 934, 424 9345, A01N 6300

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057335400

ABSTRACT:
This invention relates to the use of genetically modified, non-pathogenic bacteria on the mucosal surfaces of a host to inhibit infection by specific viruses at mucosal surfaces. Specifically, non-pathogenic bacteria are modified to acquire the capacity to bind and functionally inactivate specific viruses. Further manipulations are devised to ensure the persistent colonization of said bacteria on the desired mucosal surface of a host. The capacity to bind a pathogen by said bacteria may be accomplished through the expression on the bacterial surface of a molecule, either a polypeptide or carbohydrate moiety, which binds specifically to a molecule on the target virus. Such a capacity may be conferred upon said bacteria via genetic manipulations. Genetic manipulations of said bacteria may be carried out in vitro and the genetically-engineered bacteria applied onto the desired mucosal surface of a host, or genetic material may be directly introduced into bacteria which are already resident on the desired mucosal surface of a host through a vector.

REFERENCES:
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Int.J.Tiss REACXIII(2) 115-122 (1991), "Lactobacilli in Relation to Human Ecology and Antimicrobial Therapy," 1991 Bioscience Ediprint, Inc.
Gastroenterology 1992;102:875-878, "Fecal Recovery in Humans of Viable Bifidobacterium sp Ingested in Fermented Milk," 1992 by the American Gastroenterological Association.
Am J Clin Nutr 1992;55:78-80, "Survival bifidobacteria ingested via fermented milk during their passage through the human small intestine: an in vivo study using intestinal perfusion .sup.1-4," 1992 American Society for Clinical Nutrition.
Human Health: The Contribution of Microorganisms Formulation, Production and Marketing of Probiotic Products, "Commercial Aspects of Formulation, Production and Marketing of Probiotic Products," Chapter 10, S. Lauland.

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