Protecting nucleic acids and methods of analysis

Details Protecting nucleic acids and methods of analysis Protecting nucleic acids and methods of analysis

1996-06-06

1999-01-19

Horlick, Kenneth R.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving nucleic acid

435 912, 435 911, 536 2532, 536 173, 935 77, 935 78, C12P 1934, C07H 2100, C12N 1500

Patent

active

058612505

ABSTRACT:
A selected region of a nucleic acid is protected from attach by nuclease by completing to the nucleic acid a nucleic acid analogue of the PNA type. The surviving sequence may be detected in an assay, optionally after amplification. A PCR reaction may be sterilized and its product assayed by protecting a characterizing region of the product by PNA hybridization followed by nuclease degradation of unprotected nucleic acid.

REFERENCES:
patent: 4800159 (1989-01-01), Mullis et al.
patent: 5539082 (1996-07-01), Nielsen et al.
patent: 5578468 (1996-11-01), Pickup et al.

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