Protected binding assay avoiding non-specific protein interferen

Details Protected binding assay avoiding non-specific protein interferen Protected binding assay avoiding non-specific protein interferen

1983-04-29

1987-03-17

Marantz, Sidney

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving antigen-antibody binding, specific binding protein...

435810, 436175, 436500, 436501, 436519, 436533, 436534, 436808, 436825, G01N 3353, G01N 33546, G01N 3378

Patent

active

046507515

ABSTRACT:
A specific binding enzyme-resistant ligand assay test material, which material comprises (a) a solid phase incorporated with one partner of a specific binding pair comprising said ligand or a binding analog thereof and a specific binding protein therefor; (b) a conjugate comprising the other partner of said specific binding pair incorporated with a substance which protects the specific binding protein of said pair from enzyme inactivation when bound with its partner; and (c) an active protein-inactivating enzyme. Also a specific binding method of assaying for an enzyme-resistant ligand in a sample, which method uses the above test material and which results in a reduction in interference caused by non-specific protein.

REFERENCES:
patent: 4032626 (1977-06-01), Ward
patent: 4225576 (1980-09-01), Denning
patent: 4362531 (1982-12-01), de Steenwinkel
patent: 4427781 (1984-01-01), Masson
patent: 4455381 (1984-06-01), Magnusson
patent: 4578350 (1986-03-01), Armenta
Chemical Abstracts, 97:68911f (1982).

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