Proteases from gram positive organisms

Cleaning compositions for solid surfaces – auxiliary compositions – Cleaning compositions or processes of preparing – For cleaning a specific substrate or removing a specific...

Reexamination Certificate

Rate now

  [ 0.00 ] – not rated yet Voters 0   Comments 0

Details

C435S220000, C526S085000

Reexamination Certificate

active

07078372

ABSTRACT:
The present invention relates to the identification of a novel metalloprotease in gram positive microorganisms. The present invention provides the nucleic acid and amino acid sequences for the metalloprotease. The present invention also provides host cells having a mutation or deletion of part or all of the gene encoding the metalloprotease. The present invention provides host cells which further comprises a nucleic acid encoding desired heterologous proteins such as enzymes. The present invention also provides cleaning compositions, animal feeds and compositions used to treat a textile that include the metalloprotease of the present invention.

REFERENCES:
patent: 3817837 (1974-06-01), Rubenstein et al.
patent: 3850752 (1974-11-01), Schuurs et al.
patent: 3939350 (1976-02-01), Kronick et al.
patent: 3996345 (1976-12-01), Ullman et al.
patent: 4261868 (1981-04-01), Hora et al.
patent: 4275149 (1981-06-01), Litman et al.
patent: 4277437 (1981-07-01), Maggio
patent: 4366241 (1982-12-01), Tom et al.
patent: 4404128 (1983-09-01), Anderson
patent: 4533359 (1985-08-01), Kondo et al.
patent: 4816567 (1989-03-01), Cabilly et al.
patent: 5147642 (1992-09-01), Lotz et al.
patent: 5204015 (1993-04-01), Caldwell et al.
patent: 5264368 (1993-11-01), Ferrari et al.
patent: 5314692 (1994-05-01), Haarasilta et al.
patent: 5585253 (1996-12-01), Doi et al.
patent: 5589373 (1996-12-01), Weiner et al.
patent: 5589383 (1996-12-01), Sloma et al.
patent: 5612055 (1997-03-01), Bedford et al.
patent: 5620880 (1997-04-01), Sloma et al.
patent: 5759538 (1998-06-01), Donovan et al.
patent: 5874278 (1999-02-01), Sloma et al.
patent: 6794179 (2004-09-01), Estell
patent: 0 134 267 (1989-08-01), None
patent: 3 369 817 (1990-05-01), None
patent: 0 344 250 (1993-05-01), None
patent: 0 369 817 (1996-04-01), None
patent: WO 88/06623 (1988-09-01), None
patent: WO 89/10976 (1989-11-01), None
patent: WO 95/10615 (1995-04-01), None
patent: WO 95/14099 (1995-05-01), None
Bork, P., et al., 1994, “From genome sequences to protein function”, Current Opinion in Structural Biology, vol. 4, pp. 393-403.
UniProt Accession No. O31766, Annotation information as of Jan. 1, 1998.
PIR Accession No. G69885, Annotation information as of Oct. 15, 1999.
216034, filed Apr. 1982, RD.
*Ausubel et al., Current Protocols in Molecular Biology, John Wiley & Sons, Inc., vol. 1, Ch. 2 and 9, 1987.
*Bakhiet et al., “Studies on Transfection and Transformation of Protoplasts ofBacillus larvae, Bacillus subtilis, andBacillus popillae, ” Applied and Environmental Microbiology, vol. 49, No. 3, pp. 577-581, Mar., 1985.
*Benton et al., “Steering λgt Recombinant Clones by Hybridization to Single Plagues in situ,” Science, vol. 196, No. 4286, pp. 180-182, Apr. 8, 1977.
*Chang et al., “High Frequency Transformation ofBacillus subtilisProtoplasts by Plasmid DNA,” Molec. Gen. Genet., vol. 168, pp. 111-115, 1979.
*Contente et al., “Marker Rescue Transformation by Linear Plasmid DNA inBacillus subtilis,” Plasmid, vol. 2, pp. 555-571,1979.
*Fischer et al., “Introduction of plasmid pC194 intoBacillus thuringiensisby Protoplast transformation and plasmid transfer,” Arch of Microbiol., vol. 139, pp. 213-217, 1984.
*Grunstein et al., “Colony hybridization: A method for the isolation of cloned DNAs that contain a specific gene,” Proc. Nat. Acad. Sci. USA, vol. 72, No. 10, pp. 3961-3965, Oct., 1975.
*Haima, Peter et al., “Novel plasmid marker rescue transformation system for molecular cloning inBacillus subtilisenabling direct selection of recombinants,” Mol. Gen. Genet., vol. 223, pp. 185-191, 1990.
*Holubova et al., “Transfer of Liposome-Encapsulated Plasmid DNA toBacillus subtilisProtoplasts and Calcium-TreatedEscherichia coliCells,” Folia Microbiol., vol. 30, pp. 97-100, 1985.
*Kroll et al., “A Multifunctional Prokaryotic Protein Expression System: Overproduction, Affinity Purification, and Selective Detection,” DNA and Cell Biology, vol. 12, No. 5, pp. 441-453, 1993.
*Kunst, F. et al., “The complete genome sequence of the Gram-positive bacteriumBacillus subtilis,” Nature, vol. 390, pp. 249-264, Nov. 20, 1997.
*Kunst, F. et al., “The complete genome sequence of the Gram-positive bacteriumBacilus subtilis,” EMBL/Genbank Databases Accession No. Z9912, Sequence reference BSUB0009, Nov. 20, 1997 (XP-002115981).
*Kunst, F. et al., “ Processing proteinase homolog yfmH,” EMBL GENBANK DATABASES Accession No. G69885, Dec. 5, 1997 (XP002115982).
*Kunst F. et al., “The complete genome sequence of the Gram-positive bacteriumBacillus subtilis,” Nature, vol. 390, Nov. 20, 1997, pp. 249-256.
*Maddox et al., “Elevated Serum Levels in Human Pregnancy of a Molecule Immunochemically Similar to Eosinophil Granule Major Basic Protein,” J. Exp. Med., vol. 158, pp. 1211-1226, Oct., 1983.
*Mann et al., “Transformation ofBacillus spp.: an Examination of the Transformation ofBacillusProtoplasts by Plasmids pUB110 and pHV33,” Current Microbiology, vol. 13, pp. 191-195, 1986.
*Margot, Philippe et al., “The wprA gene ofBacillus subtilis168, expressed during exponential growth, encodes a cell-wall-associated protease,” Microbiology, vol. 142, pp. 3437-3444, 1996.
*Margot, Philippe et al., “The gene of the N-acetylglucominidase, aBacillus subtilis168 cell wall hydrolase not involved in vegetative cell autolysis,” Mol. Microbiology, vol. 12, pp. 535-545, 1994.
*McDonald et al., “Plasmid Transformation ofBacillus sphaericus1593,” Journal of General Microbiology, vol. 130, pp. 203-208, 1984.
*Murray et al., “Codon usage in plant genes,” Nucleic Acids Research, vol. 17, No. 2, pp. 477-498, 1989.
*Porath, Jerker “Immobilized Metal Ion Affinity Chromatography,” Protein Expression and Purification, vol. 3, pp. 263-281, 1992.
*Sadaie et al., “Nucleotide sequence and analysis of the phoB-rrnE-groESL region of theBacillus subtilischromosome,” Microbiology, vol. 143, pp. 1861-1866, 1997.
*Smith, Michael et al., “Protoplast Transformation in Coryneform Bacteria and Introduction of an α-Amylase Gene fromBacillus amyloliquefaciensintoBrevibacterium lactofermentum,” Applied and Environmental Microbiology, vol. 51, No. 3, pp. 634-639, Mar., 1986.
*Vorobjeva, I.P. et al., “Transformation ofBacillus megateriumProtoplasts by Plasmid DNA,” FEMS Microbiology Letters 7, pp. 261-263, 1980.
*Ward, Michael et al., “Proteinases, In Microbiol Enzymes and Biotechnology,” (W. M. Fogerty, ed), Applied Science, London, pp. 251-317, 1983.
*Weinrauch et al., “Plasmid Marker Rescue Transformation Proceeds by Breakage-Reunion inBacillus subtilis,” Journal of Bacteriology, vol. 169, No. 3, pp. 1205-1211, Mar., 1987.

LandOfFree

Say what you really think

Search LandOfFree.com for the USA inventors and patents. Rate them and share your experience with other people.

Rating

Proteases from gram positive organisms does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Proteases from gram positive organisms, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Proteases from gram positive organisms will most certainly appreciate the feedback.

Rate now

     

Profile ID: LFUS-PAI-O-3608370

  Search
All data on this website is collected from public sources. Our data reflects the most accurate information available at the time of publication.