Proteases from gram positive organisms

Details Proteases from gram positive organisms Proteases from gram positive organisms

2006-04-25

2006-04-25

Nashed, Nashaat T. (Department: 1656)

Chemistry: molecular biology and microbiology

Micro-organism, per se ; compositions thereof; proces of...

Bacteria or actinomycetales; media therefor

C435S221000, C435S320100, C435S471000, C510S300000, C536S023200

Reexamination Certificate

active

07033817

ABSTRACT:
The present invention relates to the identification of a novel metalloproteases in gram positive microorganisms. The present invention provides the nucleic acid and amino acid sequences for the metalloprotease. The present invention also provides host cells having a mutation or deletion of part or all of the gene encoding the metalloprotease. The present invention provides host cells which further comprises a nucleic acid encoding desired heterologous proteins such as enzymes. The present invention also provides cleaning compositions, animal feeds and compositions used to treat a textile that include the metalloprotease of the present invention.

REFERENCES:
patent: 3817837 (1974-06-01), Rubenstein et al.
patent: 3840466 (1974-10-01), Gray
patent: 3850752 (1974-11-01), Schuurs et al.
patent: 3939350 (1976-02-01), Kronick et al.
patent: 3996345 (1976-12-01), Ullman et al.
patent: 4261868 (1981-04-01), Hora et al.
patent: 4275149 (1981-06-01), Litman et al.
patent: 4277437 (1981-07-01), Maggio
patent: 4366241 (1982-12-01), Tom et al.
patent: 4404128 (1983-09-01), Anderson
patent: 4533359 (1985-08-01), Kondo et al.
patent: 4816567 (1989-03-01), Cabilly et al.
patent: 5147642 (1992-09-01), Lotz et al.
patent: 5204015 (1993-04-01), Caldwell et al.
patent: 5264366 (1993-11-01), Ferrari et al.
patent: 5314692 (1994-05-01), Haarasilta et al.
patent: 5411873 (1995-05-01), Adams et al.
patent: 5585253 (1996-12-01), Doi et al.
patent: 5589373 (1996-12-01), Weiner et al.
patent: 5612055 (1997-03-01), Bedford et al.
patent: 5677272 (1997-10-01), Ghosh et al.
patent: 6300117 (2001-10-01), Estell
patent: 6465186 (2002-10-01), Estell
patent: 6489108 (2002-12-01), Estell
patent: 6521440 (2003-02-01), Estell
patent: 6528255 (2003-03-01), Estell
patent: 6599731 (2003-07-01), Estell
patent: 6794179 (2004-09-01), Estell
patent: 6833265 (2004-12-01), Estell
patent: 0 134 267 (1989-08-01), None
patent: 0 369 817 (1990-05-01), None
patent: 0 344 250 (1993-05-01), None
patent: 0 590 746 (1994-04-01), None
patent: 2 031 948 (1980-04-01), None
patent: WO 88/06623 (1988-09-01), None
patent: WO 89/10976 (1989-11-01), None
patent: WO 95/10615 (1995-04-01), None
patent: WO 95/14099 (1995-05-01), None
patent: WO 99/33959 (1999-07-01), None
patent: WO 99/33960 (1999-07-01), None
patent: WO 99/34002 (1999-07-01), None
patent: WO99/34003 (1999-07-01), None
EMBL/GenBank DatabasesAccession no Z99116 ,Sequence reference BSUB0013,Nov. 20, 1997,Kunst F et al:‘The complete genome sequence of the Gram-positive bacterium Bacillus subtilis ’,XP002105783.
EMBL/GenBank Databasesaccession no F69964, Dec. 5, 1997,Kunst K et al:‘Amino acid degradation homolog ygjN’,XP002105785, * the whole document*.
Database Swiss-Prot [Online],Feb 1, 1995, Glaser P et al : “Bacillus subti lis genome project”, Database accession No. P39635, XP002252294, *abstract*.
Calogero S et al, “ROCR, A Novel Regulatory Protein Controlling Atginine Utilization In Bacillus Subtilis, Belongs To The Ntrc/Nifa Family Of Transcriptional Activators”,Journal of Bacteriology,US,vol. 176(5), Mar. 1, 1994, pp. 1234-1241, XP000670046.
Bauza T et al., “Determination of biogenic amines and their precursor amino acids in wines of the Vallee du Rhone by high-performance liquid chromatography with precolumn derivatization and fluorimetric detection”,Journal of Chromatography A, Elsevier Science,NL,vol. 707, No. 2, Jul. 21, 1995 , pp. 373-379, XP004038826.
*Kunst, F. et al., “The complete genome sequence of the Gram-positive bacteriumBacillus subtilis,” Nature, vol. 390, pp. 249-264, Nov. 20, 1997—See IDS submitted Jun. 21, 2002.
Document No. 216034, date Apr. 1982, country RD.
*Ausubel et al., Current Protocols in Molecular Biology, John Wiley & Sons, Inc. , vol. 1, Ch. 2 and 9, 1987.
*Bakhiet et al., “Studies on Transfection and Transformation of Protoplasts ofBacillus Larvae, Bacillus subtilis,andBacillus popilliae,” Applied and Environmental Microbiology, vol. 49, No. 3, pp. 577-581, Mar., 1985.
*Fischer et al., “Introduction of plasmid pC194 intoBacillus thuringiensisby Protoplast transformation and plasmid transfer,” Arch. of Microbiol., vol. 139, pp. 213-217, 1984.
*Grunstein et al., “Colony hybridization: A method for the isolation of cloned DNAs that contain a specific gene,” Proc. Nat. Acad. Sci. USA, vol. 72, No. 10, pp. 3961-3965, Oct., 1975.
*Haima, Peter et al., “Novel plasmid marker rescue transformation system for molecular cloning inBacillus subtilisenabling direct selection of recombinants,” Mol. Gen. Genet., vol. 223, pp. 185-191, 1990.
*Holubova et al., “Transfer of Liposome-Encapsulated Plasmid DNA toBacillus subtilisProtoplasts and Calcium-TreatedEscherichia coliCells,” Folia Microbiol., vol. 30, pp. 97-100, 1985.
*Kroll et al., “A Multifunctional Prokaryotic Protein Expression System: Overproduction, Affinity Purification, and Selective Detection,” DNA and Cell Biology, vol. 12, No. 5, pp. 441-453, 1993.
*Kunst, F. et al., “The complete genome sequence of the Gram-positive bacteriumBacillus subtilis,” Nature, vol. 390, pp. 249-264, Nov. 20, 1997.
*Maddox et al., “Elevated Serum Levels in Human Pregnancy of a Molecule Immunochemically Similar to Eosinophil Granule Major Basic Protein,” J. Exp. Med., vol. 158, pp. 1211-1226, Oct., 1983.
*Mann et al., “Transformation ofBacillus spp.: an Examination of the Transformation ofBacillusProtoplasts by Plasmids pUB110 and pHV33,” Current Microbiology, vol. 13, pp. 191-195, 1986.
*Margot, Philippe et al., “ThewprAgene ofBacillus subtilis168, expressed during exponential growth, encodes a cell-wall-associated protease,” Microbiology, vol. 142, pp. 3437-3444, 1996.
*Margot, Philippe et al., “The gene of the N-acetylglucominidase, aBacillus subtilis168 cell wall hydrolase not involved in vegetative cell autolysis,” Mol. Microbiology, vol. 12, pp. 535-545, 1994.
*McDonald et al., “Plasmid Transformation ofBacillus sphaericus1593,” Journal of General Microbiology, vol. 130, pp. 203-208, 1984.
*Murray et al., “Codon usage in plant genes,” Nucleic Acids Research, vol. 17, No. 2, pp. 477-498, 1989.
*Porath, Jerker “Immobilized Metal Ion Affinity Chromatography,” Protein Expression and Purification, vol. 3, pp. 263-281, 1992.
*Sadaie et al., “Nucleotide sequence and analysis of the phoB-rmE-groESL region of theBacillus subtilischromosome,” Microbiology, vol. 143, pp. 1861-1866, 1997.
*Smith, Michael et al., “Protoplast Transformation in Coryneform Bacteria and Introduction of an α-Amylase Gene fromBacillus amyloliquefaciensintoBrevibacterium lactofermentum,” Applied and Environmental Microbiology, vol. 51, No. 3, pp. 634-639, Mar., 1986.
*Vorobjeva, I.P. et al., “Transformation ofBacillus MegateriumProtoplasts by Plasmid DNA,” FEMS Microbiology Letters 7, pp. 261-263, 1980.
*Ward, Michael et al., “Proteinases, In Microbiol Enzymes and Biotechnology,” (W. M. Fogerty, ed) , Applied Science, London, pp. 251-317, 1983.
*Weinrauch et al., “Plasmid Marker Rescue Transformation Proceeds by Breakage-Reunion inBacillus subtilis,” Journal of Bacteriology, vol. 169, No. 3, pp. 1205-1211, Mar., 1987.

Affiliated with

Also associated with

Rating

Proteases from gram positive organisms does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Proteases from gram positive organisms, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Proteases from gram positive organisms will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFUS-PAI-O-3598802