Proteases from gram-positive organisms

Chemistry: molecular biology and microbiology – Micro-organism – per se ; compositions thereof; proces of... – Bacteria or actinomycetales; media therefor

Reexamination Certificate

Rate now

  [ 0.00 ] – not rated yet Voters 0   Comments 0

Details

C435S252310, C435S320100, C536S023200

Reexamination Certificate

active

06905868

ABSTRACT:
The present invention relates to the identification of novel metallo-proteases (MP) in Gram-positive microorganisms. The present invention provides the nucleic acid and amino acid sequences forBacillusMP. The present invention also provides host cells having a mutation or deletion of part or all of the gene encoding MP. The present invention also provides host cells further comprising nucleic acid encoding desired heterologous proteins such as enzymes. The present invention also provides cleaning compositions comprising an MP of the present invention.

REFERENCES:
patent: 3817837 (1974-06-01), Rubenstein et al.
patent: 3850752 (1974-11-01), Schuurs et al.
patent: 3939350 (1976-02-01), Kronick et al.
patent: 3996345 (1976-12-01), Ullman et al.
patent: 4261868 (1981-04-01), Hora et al.
patent: 4275149 (1981-06-01), Litman et al.
patent: 4277437 (1981-07-01), Maggio
patent: 4366241 (1982-12-01), Tom et al.
patent: 4404128 (1983-09-01), Anderson
patent: 4533359 (1985-08-01), Kondo et al.
patent: 4816567 (1989-03-01), Cabilly et al.
patent: 5147642 (1992-09-01), Lotz et al.
patent: 5204015 (1993-04-01), Caldwell et al.
patent: 5264366 (1993-11-01), Ferrari et al.
patent: 5314692 (1994-05-01), Haarasilta et al.
patent: 5589383 (1996-12-01), Sloma et al.
patent: 5612055 (1997-03-01), Bedford et al.
patent: 5620880 (1997-04-01), Sloma et al.
patent: 5691162 (1997-11-01), Shuster et al.
patent: 5807729 (1998-09-01), Shuster et al.
patent: 5843753 (1998-12-01), Shuster et al.
patent: 6300117 (2001-10-01), Estell
patent: 2003/0078177 (2003-04-01), Estell
patent: 21634 (1982-04-01), None
patent: 0 244 042 (1987-11-01), None
patent: 0 134 267 (1989-08-01), None
patent: 0 369 817 (1990-05-01), None
patent: WO 95/14099 (1995-05-01), None
*Ausubel, Frederick, ed. Current Protocols in Molecular Biology. John Wiley & Sons, Inc . vol. 1, Chpaters, 2, 3, and 9,1987.
*Allschul, Stephen F. et al., “Basic Local Alignment Search Tool,” J. Mol. Biol. 215:403-410,1990.
*Anagnostopoulos. C. et al., “Requirements for Transformation inBacillus Subtilis,” J. Bacteriol., 81:741-746,1961.
*Bakhiel, Nouna et al., “Studies on Transfection and Transformation of Protoplasts ofBacillus larvae, Bacilus subtilis,andBacillus popilliae,” Appl. Environ. Microbiol., vol. 49, No. 3, pp 577-581,1985.
*Benton, W. David et al., “Use of Phase Immunity in Molecular Cloning Experiments,” Science 196 180,1977.
*Bron, Sierd, “Plasmids,” Molecular Biological Methods forBacillus, ed. Harwood & Cutting, John Wiley & Sons, chapter 3,1990.
*Chang, Shing et al. “High Frequency Transformation ofBacillus subtilisProtoplasts by Plasmid DNA,” Mol. Gen. Genet. 168:111-115.1979.
*Contente, Sara et al., “Marker Rescue Transformation by Linear Plasmid DNA inBacillus subtilis,” Plasmid, 2:555-571,1979.
*Fischer, Hans-Martin et al., “Introduction of plasmid pC 194 intoBaci lus thuringiensisby protoplast transformation and plasmid transfer,” Arch. Microbiol., 139:2213-217,1981.
*Fujimura-Kamada, Konomi et al., “A Novel Membrane-associated Metalloprotease, Ste24p. Is Required for the First Step of NH:-erminal Processing of the Yeast a-Factor Precursor,” J. Cell. Biol. 136:271-285,1997.
*Grunstein, Michael et al., “Colony hybridization: A method for the isolation of cloned DNAs that contain a specific gene,” Proc. Natl. Acad Sci. U.S.A . 72:3961,1975.
Haima, Peter et al., “Novel plasmid marker rescue transformation system for molecular cloning inBacillus subtillisenabling direct selection of recombinants,” Mol. Gen Genet. 223:185-191,1990.
*Holubova, I. et al. “Transfer of Liposome-Encapsulated Plasmid DNA toBacillus subtilisProtoplasts and Calcium-TreatedEscherichia coliCells.” Folia Microbiol., vol. 30, pp. 97-100, 1985.
*Kroll, David J. et al., “A Multifunctional Prokaryotic Protein Expression System: Overproduction, Affinity Purification, and Selective Detection,” DNA Cell Biol., vol. 12, No. 5, pp. 441-453, 1993.
*Maddox, D. E. et al., “Elevated Serum Levels in Human Pregnancy of a Molecule Immunochemically Similar to Eosinophil Granule Major Basic Protein,” J. Exp. Med., vol. 158, pp. 1211-1226, Oct. 1983.
*Mann, Stephen P. et al., Transformation ofBacillusspp.: an Examination of the Transformation ofBacillusProtoplasts by Plasmids pUB 110 and pHV33, Current Microbiol., vol. 13, pp. 191-195, 1986.
*McDonald, Karen Orzech et al., “Plasmid Transformation ofBacillus sphaericus1593,” Journal of General Microbiology, vol. 130, pp. 203-208, 1984.
*Murray, Elizabeth E. et al., “Codon usage in plant genes,” Nucleic Acids Research, vol. 17, No. 2, pp. 477-498, 1989.
*Ogasawara, Naotake et al., “Systematic sequencing of theBacillus subtilisgenome: progress report of the Japanese group,” Microbiology, vol. 141, pp. 257-259, 1995.
*Porath, Jerker, “Immobilized Metal Ion Affinity Chromatography,” Protein Expression and Purification, vol. 3, pp. 263-281, 1992.
*Sambrook, J. et al., Molecular Cloning: A Laboratory Manual, 2nded., Cold Spring Harbor Laboratory Press, Cold Spring Harbor, New York, Chapters 1-3., 1989.
*Smith, Hilde et al. “Characterization of signal-sequence-coding regions selected from theBacillus subtilischromosome,” Gene, vol. 70, pp. 351-361, 1988.
*Smith, Michael D. et al. “Protoplast Transformation in Coryneform Bacteria and Introduction of an iz-Amylase Gene fromBacillus amyloliqucfaciensintoBrevibacterium lactofermentum,” Applied and Environmental Microbiology, vol. 51, No. 3, pp. 634-639, Mar., 1986.
*Vorobjeva, I P. et al., “Transformation ofBacillusMegaterium Protoplasts by Plasmid DNA,” FEMS Microbiology Letters, vol. 7, pp. 261-263, 1980.
Ward, Owen P., “Proteinases,” Microbial Enzymes and Biotechnology, William Fogarty, ed., Applied Science Publishers, London and New York, pp. 251-317, 1983.
*We nrauch, r. et al. “Plasmid Marker Rescue Transformation inBacillus subtilis.” Journal of Bacteriology, vol. 154, No. 3, pp. 1077-1087, Jun., 1983.
*Weinrauch, r. et al., “Plasmid Marker Rescue Transformation Proceeds by Breakage-Reunion inBacillus subtilis.” Journal of Bacteriology, vol. 169, No. 3, pp. 1205-1211, Mar. 1987.
*EMBL/GenBank: Databases Accession No. Y14083, Sequence reference BSY14083, XP002094692.

LandOfFree

Say what you really think

Search LandOfFree.com for the USA inventors and patents. Rate them and share your experience with other people.

Rating

Proteases from gram-positive organisms does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Proteases from gram-positive organisms, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Proteases from gram-positive organisms will most certainly appreciate the feedback.

Rate now

     

Profile ID: LFUS-PAI-O-3522845

  Search
All data on this website is collected from public sources. Our data reflects the most accurate information available at the time of publication.