Chemistry: molecular biology and microbiology – Enzyme – proenzyme; compositions thereof; process for... – Hydrolase
Patent
1991-04-05
1993-04-06
Naff, David M.
Chemistry: molecular biology and microbiology
Enzyme , proenzyme; compositions thereof; process for...
Hydrolase
435814, 435 23, 424 9464, C12N 948, C12Q 137, A61K 37547
Patent
active
052003396
ABSTRACT:
A proteolytic factor is capable of cleaving .beta.-protein precursor at a site near the .beta.-protein N-terminus. Also, a method for treating Alzheimer's disease in a patient includes steps of reducing .beta.-protein precursor proteolysis outside the .beta.-protein domain at a site near the .beta.-protein N-terminus. Also, a method for purifying an enzyme from a sample includes steps of incubating the sample with a labelled substrate of the enzyme or with a labelled fragment of a substrate to which the enzyme binds, treating the sample with a crosslinking agent to crosslink any enzyme-substrate complexes in the sample, and recovering labelled complexes. Also, a method for diagnosis in a subject of a disease characterized by accumulation of amyloid includes determining the level, in a sample of tissue or body fluid from the subject, of an AD proteolytic factor. Also, a method for screening for an agent useful in treatment of a disease characterized by accumulation of amyloid includes steps of incubating an AD protease with a peptide having an amino acid sequence corresponding to the sequence spanning the .beta.-protein N-terminus in the presence of a candidate agent, and determining the degradation of the peptide.
REFERENCES:
Nelson et al., (1990) J. Biol. Chem., 265(7), 3836-3843.
Nelson et al. (1989) Soc. Neurosci. Abst., 15, 330, Abst. #135.9.
Abraham et al. (1991) Biochem. Biophys. Res. Comm., 174(2), 790-796.
Siman et al. (1990) J. Neurosci., 10(7), 2400-2410.
Barrett (1981) Meth. Enzymol., 80, 561-564, Acad. Press, N.Y.
Cataldo et al. (1990) Proc. Nat. Acad. Sci. USA, 87, 3861-3865.
Matsumoto et al. (1991) Biochem. Biophys. Res. Comm., 175(2), 361-365.
Justice et al. (1987) J. Cell. Biochem., 34, 227-238.
Abraham et al. (1989) Cell, 52, 487-501.
Bowen, et al., "Cathepsin A in Human Brain and Spleen", 1973, Biochem. J., vol. 131, pp. 417-419.
Naff David M.
Weber Jon P.
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