Protease specific cleavable luciferases and methods of use...

Details Protease specific cleavable luciferases and methods of use... Protease specific cleavable luciferases and methods of use...

2005-05-10

2005-05-10

Achutamurthy, Ponnathapu (Department: 1652)

Chemistry: molecular biology and microbiology

Enzyme , proenzyme; compositions thereof; process for...

Oxidoreductase

C435S212000, C435S440000, C536S023200, C536S023100, C536S023400

Reexamination Certificate

active

06890745

ABSTRACT:
Provided are methods and compositions useful in detecting protease activity in a sample, as well as methods of identifying agents that modulate protease activity. The methods and compositions provide a modified luciferase polynucleotide sequence and a luciferase polypeptide containing protease recognition sequences, wherein cleavage of the recognition sequence by a protease inhibits luciferase activity. Further provided are methods and compositions for detecting and modulating caspase activity and apoptosis.

REFERENCES:
patent: WO 9814605 (1998-04-01), None
Korant et al. Cleavage of Cellular Proteins by the HIV-1 Protease. Proteolysis in Cell Functions. (1997), 13, pp. 520-523.*
Thompson et al. The Journal of Biological Chemistry, vol. 272, No. 30, pp. 18766-18771, 1997.*
Lorenz et al. J. Biolumin Chemlumin, vol. 11, pp. 31-37, 1996.*
Xu et al. Nucleci Acids Research, vol. 26, No. 8, pp. 2034-2035, 1998.*
Korant et al., “Clevage of Cellular Proteins by the HIV-1 Protease”,Biochemical and Health Research, 1997, pp. 520-523.
Wang et al., “TheRenillaLuciferase-Modified GFP Fusion Protein is Functional in Transformed Cells”Biohydrogen, ‘Proceedings of an International Conference on Biological Hydrogen Production’ Waikoloa, HI, Jun. 23-26, 1997.
Liu et al., “Visualizing and Quantifying Protein Secretion Using aRenillaLuciferase-GFP Fusion Protein”,Luminescence(Chichester), vol. 15, No. 1, Jan. 2000, pp. 45-49.
Leng et al., “CleavaLite™ : A Novel Bioluminescent Caspase Activity Assay”,Society for Neuroscience Abstracts, vol. 27, No. 2, 2001, p. 1555.

Affiliated with

Also associated with

Rating

Protease specific cleavable luciferases and methods of use... does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Protease specific cleavable luciferases and methods of use..., we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Protease specific cleavable luciferases and methods of use... will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFUS-PAI-O-3417413