Protease inactivated .alpha.-amylase preparations

Details Protease inactivated .alpha.-amylase preparations Protease inactivated .alpha.-amylase preparations

1976-08-05

1980-11-25

Shapiro, Lionel M.

Chemistry: molecular biology and microbiology

Enzyme , proenzyme; compositions thereof; process for...

Hydrolase

435813, 435836, C12N 928

Patent

active

042359708

ABSTRACT:
Protease enzyme impurities contained in bacterial .alpha.-amylase enzyme preparations are inactivated by a mild heat treatment in the presence of a protective material. Useful protective materials include calcium and starch hydrolysates such as corn syrup. The protease-free .alpha.-amylase can then be used to solubilize starch materials by various granular starch and conventional processes. Hydrolysates obtained contain significantly less soluble protein than those prepared using untreated .alpha.-amylases. A preferred .alpha.-amylase enzyme preparation is one derived from a Bacillus licheniformis microorganism.

REFERENCES:
patent: 2683682 (1919-07-01), Miller et al.
patent: 3039936 (1962-06-01), Lenney et al.
patent: 3249512 (1966-05-01), Bode
patent: 3303102 (1967-02-01), Armbruster
patent: 3819528 (1974-06-01), Berry
patent: 3912590 (1975-10-01), Slott et al.

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