Chemistry: molecular biology and microbiology – Enzyme – proenzyme; compositions thereof; process for... – Hydrolase
Patent
1991-05-24
1993-05-25
Naff, David M.
Chemistry: molecular biology and microbiology
Enzyme , proenzyme; compositions thereof; process for...
Hydrolase
435859, 435219, C12N 952, C12N 950, C12N 100
Patent
active
052139786
ABSTRACT:
An enzyme material isolated from a culture of Micrococcus sedentarius which comprises one or more proteases having an ability to degrade protein, including human callus. In one embodiment the enzyme material is water soluble, non-dialyzable through a membrane having a molecular weight cut-off of 10 kDa, has an isoelectric point of 4.6, a molecular weight of 30.3 kDa, an optimum pH for protease activity at about 8.2, and functions at an optimum temperature of about 40.degree. C. In another embodiment, the enzyme material is water-soluble, non-dialyzable through a membrane having a molecular weight cut off of 10 kDa, has an isoelectric point of 2.7, a molecular weight of 50 kDa, an optimum pH for protease activity at about pH 10.2, and functions at an optimum temperature of about 46.degree. C.
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Meller Mike
Naff David M.
Scholl PLC
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