Protease EFC cell surface fusion protein assay

Details Protease EFC cell surface fusion protein assay Protease EFC cell surface fusion protein assay

2003-01-28

2008-11-18

Kam, Chih-Min (Department: 1656)

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving hydrolase

C435S024000, C435S014000, C435S018000, C435S004000, C435S069700, C435S069800, C435S070100, C435S173400

Reexamination Certificate

active

07452690

ABSTRACT:
Methods and reagents are provided for measuring protease activity. The reagent comprises a surface to which is linked an enzyme donor fragment through a protease recognition sequence, where the enzyme donor fragment complexes with an enzyme acceptor fragment to form an active indicator enzyme when the enzyme donor fragment is cleaved from said surface. Conveniently, the surface is a cell membrane surface where the reagent is expressed in the cell. The method comprises bringing together the reagent, the protease, the enzyme acceptor and substrate for the indicator enzyme and measuring the indicator enzyme activity as a measure of the protease activity. The method finds application in screening for compounds modulating the activity of the protease.

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