Protease deletion

Details Protease deletion Protease deletion

1990-09-07

1997-04-15

Jacobson, Dian C.

Chemistry: molecular biology and microbiology

Treatment of micro-organisms or enzymes with electrical or...

Modification of viruses

43525231, 4352525, 435222, 424 932, C12N 954, C12N 121, C12N 1557

Patent

active

056208804

ABSTRACT:
A Bacillus cell contains a mutation in the epr gene resulting in inhibition of the production by the cell of the proteolytically active epr gene product; the cell may further contain mutations in the genes encoding proteolytically active residual protease I (RP-I) and proteolytically active residual protease II (RP-II).

REFERENCES:
patent: 4828994 (1989-05-01), Fahnestock et al.
Fahnestock, S.R., et al. Appl. & Environ. Micro. 53:379-384 (1987).
Roitsch, C.A. et al. "Bacillopeptidase F: Two Forms of a Glycoprotein Serine Protease from Bacillus subtilis 168." J. Bacteriol. 155(1):148-152 (1983).
Stahl, M.L. et al. "Replacement of the B. subtilis Subtilisin Structural Gene with an in Vitro-Derived Mutation." J.Bacteriol. 158(2)411-418 (1984).
Yang, M.Y. et al. "Cloning of the Neutral Protease Gene of B. subtilis." J. Bacteriol. 160(1):15-21 (1984).
Ferrari, E. et al. "Effect of Stage O Sporulation Mutations on Subtilisin Expression." J.Bacteriol. 166(1):173-179 (1986).
Koide, Y. et al. "Cloning & Sequencing of the Major Intracellular Serine Protease Gene of Bacillus subtilis," J.Bacteriol. 167(1):110-116 (1986).
Br uckner, R. et al. "Characterization of a Gene for a Minor Extra-Cellular Protease from Bacillus subtilis, " Fourth International Conference on Genetics & Biotech. of Bacilli, T-13 (1987).

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