Protease deletion

Details Protease deletion Protease deletion

1997-04-15

1999-02-23

Jacobson, Dian C.

Chemistry: molecular biology and microbiology

Enzyme , proenzyme; compositions thereof; process for...

Hydrolase

4352525, 4353201, 536 232, C12N 121, C12N 956, C12N 1557

Patent

active

058742786

ABSTRACT:
A Bacillus cell contains a mutation in the epr gene resulting in inhibition of the production by the cell of the proteolytically active epr gene product; the cell may further contain mutations in the genes encoding proteolytically active residual protease I (RP-I) and proteolytically active residual protease II (RP-II) (mpr).

REFERENCES:
patent: 4266031 (1981-05-01), Tang et al.
patent: 4828994 (1989-05-01), Fahnestock et al.
patent: 5585253 (1996-12-01), Doi
Akparov et al., "Chemical Abstracts," 98, 67639g (1983).
Band et al., "Construction and Properties of an Intracellular Serine Protease Mutant of Bacillus subtilis," Journal of Bacteriology, 169:444-446, 1987.
Beall et al., Cloning and Characterization of Bacillus subtilis Homologs of Escherichia coli Cell Division Genes ftsZ and ftsA, Journal of Bacteriology, 170:4855-4864, 1988.
Bruckner and Doi, Characterization of a Gene for Minor Extracellular Protease from Bacillus subtilis, Abstract, No. 13, Fourth Conf. on Genetics and Biotechnology of Bacilli, San Diego, CA, 1987.
Bruckner et al., Characterization of a Gene for a Minor Extracellular Protease of Bacillus subtilis Produced During Sporulation, Abstract, No. 49, Program of the Tenth Int'l Spores Conf., Woods Hole, MA, 1988.
Bruckner et al., Multiple Active Forms of a Novel Serine Protease From Bacillus subtilis, Mol. Gen Genet, 221:486-490, 1990.
Bruckner et al. "Characterization of a Gene for a Minor Extra-Cellular Protease from Bacillus subtilis," Fourth International Conference on Genetics & Biotech. of Bacilli, T-13 (1987).
Creighton, "Proteins", W.H. Freeman and Company New York, pp. 93-131 (1984).
Fahnestock and Fisher, Protease-Deficient Bacillus subtilis Host Strains for Production of Staphylococcal Protein A, Applied and Environmental Microbiology 53:379-384, 1987.
Fahnestock, S.R. et al., Appl. & Environ. Micro., 53:379-384 (1987).
Ferrari et al., Effect of Stage O Sporulation Mutations on Subtilisin Expression, Journal of Bacteriology, 166:173-179, 1986.
Kawamura and Doi, "Construction of a Bacillus subtilis Double Mutant Deficient in Extracellular Alkaline and Neutral Proteases," J. Bacteriol., 160:442-444, 1984.
Koide et al., "Cloning and Sequencing of the Major Intracellular Serine-Protease Gene of Bacillus subtilis," J. Bacteriol. 167:110-116, 1986.
Niidome et al., J. Biochem 108:965-970 (1990).
Novo Leaflet "Alcalase.RTM." (1982).
Ohmura et al., A Bacillus subtilis Secretion Vector System Derived from the B. subtilis A-Amylase Promoter and Signal Sequence Region, and Secretion, J. Biochem., 95:87-93, 1984.
Palva et al., Secretion of Interferon by Bacillus subtilis, Gene 22:229-235, 1983.
Roitsch and Hageman, Bacillopeptidase F: Two Forms of a Glycoprotein Serine Protease From Bacillus subtilis, J. of Bacteriology 155:152, 1983.
Roitsch, C.A. et al., "Bacillopeptidase F: Two Forms of A Glycoprotein Serine Protease from Bacillus subtilis 168." J. Bacteriol. 155(1):148-152 (1983).
Rufo et al., Isolation and Characterization of a Novel Extracellular Metalloprotease From Bacillus subtilis, Journal of Bacteriology 172:1019-1023, 1990.
Sloma et al., Gene Encoding a Minor Extracellular Protease in Bacillus subtilis, J. of Bacteriology, 170-5557-5563, 1988.
Sloma et al., Bacillopeptidase F of Bacillus subtilis: Purification of the Protein and Cloning of the Gene J. of Bacteriology 172:1470-1477, 1990.
Sloma et al., Bacillopeptidase F of Bacillus subtilis: Purification of the Protein and Cloning of the Gene, Errata, J. Bact. 172:5520-5521, Sep. 1990.
Sloma et al., Gene Encoding a Novel Extracellular Metalloprotease in Bacillus subtilis, J. of Bacteriology 172:1024-1029, 1990.
Smith et al, Characterization of Signal-Sequence-Coding Regions Selected From the Bacillus subtilis Chromosome, Gene 70:351-361, 1988.
Srivastava and Aronson, Isolation and Characterization of a Unique Protease from Sporulating Cells of Bacillus subtilis, Arch Microbiol 129:227-232, 1981.
Stahl and Ferrari, Replacement of the Bacillus subtilis Subtilisin Structural Gene with an In Vitro-Derived Deletion Mutation, J. of Bacteriology 158:411-418, 1984.
Verbruggen, Biochem. J. 151:149-155 (1975).
Vitkovic et al., J. Bacteriol. 131:891-896 (1977).
Wang et al., Construction of Bacillus subtilis Mutant-Deficient in Three Extracellular Proteases, J. Gen. Appl. Microbiol. 35:487-492, 1989.
Wu et al., Cloning Genetic Organization, and Characterization of a Structural Gene Encoding Bacillopeptidase F From Bacillus subtilis, The J. of Biological Chemistry 265:6845-6850, 1990.
Yang et al., Cloning of the Neutral Protease Gene of Bacillus subtilis and the Use of the Cloned Gene to Create an In Vitro-Derived Deletion Mutation, J. of Bacteriology, 160:15-21, 1984.
Yoshida et al., J. Biochem. 104:451-456 (1988).

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