Protease-deficient strains of Pichia methanolica

Details Protease-deficient strains of Pichia methanolica Protease-deficient strains of Pichia methanolica

1999-03-09

2000-11-28

Horlick, Kenneth R.

Chemistry: molecular biology and microbiology

Micro-organism, per se ; compositions thereof; proces of...

Fungi

43525423, 435 7001, 435219, 435 691, 435 6, 536 2374, 536 241, 935 28, 935 37, 935 69, C12N 114

Patent

active

061534242

ABSTRACT:
Protease-deficient strains of the methylotrophic yeast Pichia methanolica and materials and methods for generating such strains are disclosed. The strains have a functional deficiency in a vacuolar protease, such as proteinase A or proteinase B. The strains are useful as hosts for the expression of heterologous genes encoding proteins of commercial or other interest.

REFERENCES:
patent: 5324660 (1994-06-01), Gleeson et al.
patent: 5541112 (1996-07-01), Gleeson et al.
patent: 5792850 (1998-08-01), Baumgartner
Raymond et al., Yeast 14:11-23, 1998.

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