Protease assays

Details Protease assays Protease assays

1997-07-23

1999-04-06

Wax, Robert A.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving nucleic acid

435 23, 435 697, 435212, 4352523, 4353201, 530350, 530358, 530371, 536 232, 536 234, 536 2372, 536 2374, 536 241, C12Q 168, C12Q 137, A61K 3816, C07H 2104

Patent

active

058916350

ABSTRACT:
Hybrid regulatory proteins are provided which contain amino acid sequences that are susceptible to cleavage by specific proteolytic enzymes. When acted upon by such enzymes, the hybrid regulatory proteins are rendered substantially less active, thereby altering the rate of production of products of indicator genes that are controlled by the regulatory proteins. Also provided are DNAs encoding such regulatory proteins, recombinant vectors and transformed eukaryotic cells containing such DNAs, and methods for identifying inhibitors of the specific proteolytic enzymes.

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