Protease assays

Details Protease assays Protease assays

1994-11-03

1997-02-04

Low, Christopher S. F.

Chemistry: natural resins or derivatives; peptides or proteins;

Proteins, i.e., more than 100 amino acid residues

530371, 530358, 53038826, 5303875, 435 691, 435 697, 435 699, 930310, 930240, C07K 1900, C07K 14395

Patent

active

055999069

ABSTRACT:
Hybrid regulatory proteins are provided which contain amino acid sequences that are susceptible to cleavage by specific proteolytic enzymes. When acted upon by such enzymes, the hybrid regulatory proteins are rendered substantially less active, thereby altering the rate of production of products of indicator genes that are controlled by the regulatory proteins. Also provided are DNAs encoding such regulatory proteins, recombinant vectors and transformed eukaryotic cells containing such DNAs, and methods for identifying inhibitors of the specific proteolytic enzymes.

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