Protease and process for production and use thereof

Details Protease and process for production and use thereof Protease and process for production and use thereof

1985-04-12

1987-03-17

Shapiro, Lionel M.

Chemistry: molecular biology and microbiology

Enzyme , proenzyme; compositions thereof; process for...

Hydrolase

435219, 435 69, 435942, C12N 960, C12N 950, C12P 2106, C12R 1865

Patent

active

046507639

ABSTRACT:
Disclosed is a new protease having the following properties: (1) it is able to hydrolitically cleave a peptide bond between two adjacent basic amino acids in a peptide chain; (2) it has a molecular weight of about 43,000 as determined by electrophoresis; (3) it is inhibited by phenylmethylsulphonyl fluoride and diisopropyl fluorophosphate, but is not inhibited by monoiodoacetate, p-chloromercuribenzoic acid, ethylenediaminetetraacetic acid, 1,10-phenanthroline, tosyl-L-lysine chloromethyl ketone, and leupeptin. The protease can be produced by culturing Saccharomyces cerevisiae, and recovering purification by conventional methods, and is useful as a processing enzyme for conversion of a prohormone to an active hormone.

REFERENCES:
Kurjan, J. et al., Cell, 30, 933-943, (1982).
Julius, D. et al., Cell, 32, 839-852, (1983).
Fletcher et al., J. Cell. Biol., 90, 312-322, (1981).
Loh, Y. P. et al., Proc. Natl. Acad. Sci. U.S.A., 79, 108-112, (1982).
Mizuno et al., Biochem. Biophys. Res. Commun., 108, 1235-1242.
Lindberg, I. et al., Biochem. Biophys. Res. Commun., 106, 186-193, (1982).
Evangelista, R. et al., Biochem. Biophys. Res. Commun., 106, 895-902, (1982).
Fricker, L. D. et al., Proc. Natl. Acad. Sci. U.S.A., 79, 3886-3890, (1982).

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